Molecular characterization of gene sequences coding for protein disulfide isomerase (PDI) in durum wheat (Triticum turgidum ssp durum)

被引:31
作者
Ciaffi, M [1 ]
Paolacci, AR [1 ]
Dominici, L [1 ]
Tanzarella, OA [1 ]
Porceddu, E [1 ]
机构
[1] Univ Tuscia, Dept Agrobiol & Agrochem, I-01100 Viterbo, Italy
关键词
gene structure; gene expression; pre-mRNA splicing; protein domain; binding protein (BiP);
D O I
10.1016/S0378-1119(01)00348-1
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The organisation of the durum wheat genomic sequence (3.5 kb) coding for protein disulfide isomerase (PDI), deduced by comparison between genomic fragments and cDNA sequences (1.5 kb) isolated from immature caryopses, is described. The gene structure consists of ten exons and nine introns. The presence of consensus sequences involved in splicing, such as intron-exon junctions and branchpoint, has been observed and discussed. Although the deduced wheat PDI amino acid sequence exhibited an overall identity of only 31% to that of human PDI, their modular architecture in terms of number, size, location and secondary structure-propensities of the constituent domains are remarkably similar. The comparison of the amino acid sequences with the eight available plant PDI-like sequences showed a high identity with four of them and low with the remaining ones. Analyses of transcription levels showed that the PDI mRNA was present in all analysed tissues, with much higher expression in immature caryopses. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:147 / 156
页数:10
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