Pluripotency and Cellular Reprogramming: Facts, Hypotheses, Unresolved Issues

被引：591

作者：

Hanna, Jacob H. ^{[1
]}

Saha, Krishanu ^{[1
]}

Jaenisch, Rudolf ^{[1
,2
]}

机构：

[1] MIT, Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[2] MIT, Dept Biol, Cambridge, MA 02142 USA

来源：

CELL | 2010年 / 143卷 / 04期

关键词：

EMBRYONIC STEM-CELLS; GENE-EXPRESSION; DNA METHYLATION; HOMOLOGOUS RECOMBINATION; GROUND-STATE; T-CELLS; B-CELLS; FIBROBLASTS; GENERATION; DERIVATION;

D O I：

10.1016/j.cell.2010.10.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Direct reprogramming of somatic cells to induced pluripotent stem cells by ectopic expression of defined transcription factors has raised fundamental questions regarding the epigenetic stability of the differentiated cell state. In addition, evidence has accumulated that distinct states of pluripotency can interconvert through the modulation of both cell-intrinsic and exogenous factors. To fully realize the potential of in vitro reprogrammed cells, we need to understand the molecular and epigenetic determinants that convert one cell type into another. Here we review recent advances in this rapidly moving field and emphasize unresolved and controversial questions.

引用

页码：508 / 525

页数：18

共 99 条

[1]

Epigenetic reversion of post-implantation epiblast to pluripotent embryonic stem cells [J].

Bao, Siqin ;

Tang, Fuchou ;

Li, Xihe ;

Hayashi, Katsuhiko ;

Gillich, Astrid ;

Lao, Kaiqin ;

Surani, M. Azim .

NATURE, 2009, 461 (7268) :1292-1295

[2]

Reprogramming towards pluripotency requires AID-dependent DNA demethylation [J].

Bhutani, Nidhi ;

Brady, Jennifer J. ;

Damian, Mara ;

Sacco, Alessandra ;

Corbel, Stephane Y. ;

Blau, Helen M. .

NATURE, 2010, 463 (7284) :1042-U57

[3]

Evolving strategies for enzyme engineering [J].

Bloom, JD ;

Meyer, MM ;

Meinhold, P ;

Otey, CR ;

MacMillan, D ;

Arnold, FH .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2005, 15 (04) :447-452

[4]

Oct4 distribution and level in mouse clones:: consequences for pluripotency [J].

Boiani, M ;

Eckardt, S ;

Schöler, HR ;

McLaughlin, KJ .

GENES & DEVELOPMENT, 2002, 16 (10) :1209-1219

[5]

Adult mice generated from induced pluripotent stem cells [J].

Boland, Michael J. ;

Hazen, Jennifer L. ;

Nazor, Kristopher L. ;

Rodriguez, Alberto R. ;

Gifford, Wesley ;

Martin, Greg ;

Kupriyanov, Sergey ;

Baldwin, Kristin K. .

NATURE, 2009, 461 (7260) :91-U94

[6]

Core transcriptional regulatory circuitry in human embryonic stem cells [J].

Boyer, LA ;

Lee, TI ;

Cole, MF ;

Johnstone, SE ;

Levine, SS ;

Zucker, JR ;

Guenther, MG ;

Kumar, RM ;

Murray, HL ;

Jenner, RG ;

Gifford, DK ;

Melton, DA ;

Jaenisch, R ;

Young, RA .

CELL, 2005, 122 (06) :947-956

[7]

Derivation of pluripotent epiblast stem cells from mammalian embryos [J].

Brons, I. Gabrielle M. ;

Smithers, Lucy E. ;

Trotter, Matthew W. B. ;

Rugg-Gunn, Peter ;

Sun, Bowen ;

de Sousa Lopes, Susana M. Chuva ;

Howlett, Sarah K. ;

Clarkson, Amanda ;

Ahrlund-Richter, Lars ;

Pedersen, Roger A. ;

Vallier, Ludovic .

NATURE, 2007, 448 (7150) :191-U7

[8]

A Murine ESC-like State Facilitates Transgenesis and Homologous Recombination in Human Pluripotent Stem Cells [J].

Buecker, Christa ;

Chen, Hsu-Hsin ;

Polo, Jose Maria ;

Daheron, Laurence ;

Bu, Lei ;

Barakat, Tahsin Stefan ;

Okwieka, Patricia ;

Porter, Andrew ;

Gribnau, Joost ;

Hochedlinger, Konrad ;

Geijsen, Niels .

CELL STEM CELL, 2010, 6 (06) :535-546

[9]

Capture of Authentic Embryonic Stem Cells from Rat Blastocysts [J].

Buehr, Mia ;

Meek, Stephen ;

Blair, Kate ;

Yang, Jian ;

Ure, Janice ;

Silva, Jose ;

McLay, Renee ;

Hall, John ;

Ying, Qi-Long ;

Smith, Austin .

CELL, 2008, 135 (07) :1287-1298

[10]

Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome [J].

Carvajal-Vergara, Xonia ;

Sevilla, Ana ;

D'Souza, Sunita L. ;

Ang, Yen-Sin ;

Schaniel, Christoph ;

Lee, Dung-Fang ;

Yang, Lei ;

Kaplan, Aaron D. ;

Adler, Eric D. ;

Rozov, Roye ;

Ge, YongChao ;

Cohen, Ninette ;

Edelmann, Lisa J. ;

Chang, Betty ;

Waghray, Avinash ;

Su, Jie ;

Pardo, Sherly ;

Lichtenbelt, Klaske D. ;

Tartaglia, Marco ;

Gelb, Bruce D. ;

Lemischka, Ihor R. .

NATURE, 2010, 465 (7299) :808-U12

← 1 2 3 4 5 6 7 8 9 10 →