Monoclonal antibody purification by affinity chromatography with ligands derived from the screening of peptide combinatory libraries

被引：34

作者：

Camperi, SA

Iannucci, NB

Albanesi, GJ

Eberhardt, MO

Etcheverrigaray, M

Messeguer, A

Albericio, F

Cascone, O

机构：

[1] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Microbiol Ind & Biotecnol, RA-1113 Buenos Aires, DF, Argentina

[2] Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Lab Cultivos Celulares, Santa Fe, Argentina

[3] CSIC, Inst Invest Bioquim & Ambientales, Barcelona, Spain

[4] Univ Barcelona, Fac Quim, Dept Quim Organ, Barcelona, Spain

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 18期

关键词：

affinity chromatography; combinatorial library; monoclonal antibody purification; peptide ligands;

D O I：

10.1023/A:1025464918453

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix obtained by attachment of APAR to agarose, having a peptide density of 0.5 mumol ml(-1), showed a maximum capacity of 9.1 mg Mab ml(-1) and a dynamic capacity of 3.9 mg Mab ml(-1). A 95% yield of electrophoretically pure anti-GM-CSF was obtained in a single step.

引用

页码：1545 / 1548

页数：4

共 12 条

[1]

BAUMBACH GA, 1993, J CELL BIOCH SA, V17, P43

[2] PREDICTION OF THE PERFORMANCE OF PREPARATIVE AFFINITY-CHROMATOGRAPHY [J].

CHASE, HA .

JOURNAL OF CHROMATOGRAPHY, 1984, 297 (AUG) :179-202

[3] PEPTIDES ON PHAGE - A VAST LIBRARY OF PEPTIDES FOR IDENTIFYING LIGANDS [J].

CWIRLA, SE ;

PETERS, EA ;

BARRETT, RW ;

DOWER, WJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) :6378-6382

[4] Identification of two potential receptor-binding sites for hGM-CSF [J].

Eberhardt, MO ;

Frank, R ;

Kratje, R ;

Etcheverrigaray, M .

BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, 2003, 20 (01) :15-20

[5]

Hermanson G.T., 1992, Immobilized affinity ligand techniques

[6] Identification of peptides mimicking the antigenicity and immunogenicity of conformational epitopes on Japanese encephalitis virus protein using synthetic peptide libraries [J].

Hirabayashi, Y ;

Fukuda, H ;

Kimura, J ;

Miyamoto, M ;

Yasui, K .

JOURNAL OF VIROLOGICAL METHODS, 1996, 61 (1-2) :23-36

[7]

Huang PY, 1999, BIOTECHNOL BIOENG, V63, P633, DOI 10.1002/(SICI)1097-0290(19990620)63:6<633::AID-BIT1>3.3.CO

[8]

2-3

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] A NEW TYPE OF SYNTHETIC PEPTIDE LIBRARY FOR IDENTIFYING LIGAND-BINDING ACTIVITY [J].

LAM, KS ;

SALMON, SE ;

HERSH, EM ;

HRUBY, VJ ;

KAZMIERSKI, WM ;

KNAPP, RJ .

NATURE, 1991, 354 (6348) :82-84

← 1 2 →