Fluorescence microscopy study of protein adsorption at modified glassy carbon surfaces

被引：20

作者：

Downard, AJ ^{[1
]}

Jackson, SL

Tan, ESQ

机构：

[1] Univ Canterbury, Dept Chem, MacDiarmid Inst Adv Mat & Nanotechnol, Christchurch 1, New Zealand

[2] Univ Canterbury, Sch Biol Sci, Christchurch 1, New Zealand

来源：

AUSTRALIAN JOURNAL OF CHEMISTRY | 2005年 / 58卷 / 04期

关键词：

D O I：

10.1071/CH04259

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Glassy carbon (GC) surfaces were modified with thin films by means of electrochemically assisted reduction of aryl diazonium salts and oxidation of primary amines. GC plates with modified and unmodified areas were exposed to solutions of fluorescently labelled bovine serum albumin (BSA-FITC). Fluorescence microscopy was found to be a simple and direct method for comparing adsorption of BSA-FITC between the different areas. Modification with methylbenzene, hexylbenzene, poly( ethylene glycol) benzene, and tetraethylene glycol diamine groups increases protein adsorption relative to unmodified GC. Hexylamine and the poly( ethylene glycol) diamine ED-2003 reduce protein adsorption. The results give insight into some factors controlling protein adsorption at these surface films.

引用

页码：275 / 279

页数：5

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