Label-Free Biosensors Based on Aptamer-Modified Graphene Field-Effect Transistors

被引：429

作者：

Ohno, Yasuhide ^{[1
]}

Maehashi, Kenzo ^{[1
]}

Matsumoto, Kazuhiko ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Osaka 5670047, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 51期

基金：

日本科学技术振兴机构;

关键词：

IN-VITRO SELECTION; MOLECULES; LIGANDS;

D O I：

10.1021/ja108127r

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A label-free immunosensor based on an aptamer-modified graphene field-effect transistor (G-FET) is demonstrated. Immunoglobulin E (IgE) aptamers with an approximate height of 3 nm were successfully immobilized on a graphene surface, as confirmed by atomic force microscopy. The aptamer-modified G-FET showed selective electrical detection of IgE protein. From the dependence of the drain current variation on the IgE concentration, the dissociation constant was estimated to be 47 nM, indicating good affinity and the potential for G-FETs to be used in biological sensors.

引用

页码：18012 / 18013

页数：2

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