Silver nitrate:: Antimicrobial activity related to cytotoxicity in cultured human fibroblasts

被引:64
作者
Hidalgo, E
Bartolomé, R
Barroso, C
Moreno, A
Domínguez, C
机构
[1] Univ Autonoma Barcelona, Ctr Invest Bioquim & Biol Mol, E-08193 Barcelona, Spain
[2] Univ Autonoma Barcelona, Serv Farm Hosp Maternoinfantil, E-08193 Barcelona, Spain
[3] Univ Autonoma Barcelona, Serv Microbiol & Parasitol, Vall dHebron Hosp, E-08193 Barcelona, Spain
来源
SKIN PHARMACOLOGY AND APPLIED SKIN PHYSIOLOGY | 1998年 / 11卷 / 03期
关键词
antimicrobial; topical antiseptics; cell viability; cultured fibroblasts; cytotoxicity; silver; XXT assay;
D O I
10.1159/000029820
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
The aims of this study were to ascertain whether silver nitrate (AgNO3) concentrations below those used in clinical practice inhibit bacterial growth, and in parallel study the cytotoxic effects on human fibroblasts. The cytoprotective effects of fetal calf serum (FCS) were also evaluated, The cytotoxic effects of Fight different silver nitrate concentrations R-ere determined by assessing mitochondrial activity of viable cells capable of cleaving tetrazolium salts. Antimicrobial activity of AgNO3, range: 7-550 x I0(-5) %, was tested against Staphylococcus aureus, Citrobacter freundii, and Pseudomonas aeruginosa. Silver nitrate concentrations exerting antimicrobial effects were: S. aureus, >70 x 10(-5) %; P. aeruginosa, greater than or equal to 270 x 10(-5) %, and C. freundii, greater than or equal to 550 x 10(-5) %. With 2% FCS, the lowest AgNO3 concentration studied(7 x 10(-5) %) showed cytotoxic effects (cell survival 71 +/- 19%) at only 2 h of incubation. Under these conditions AgNO3 cytotoxicity was time- and concentration-dependent in all exposure periods. Cytotoxicity was greatly enhanced causing 76% fibroblast growth inhibition ar concentrations of 14 x 10(-5) % and contact lime of 2 h, The AgNO3 concentration of 7 x 10(-5) % was also cytotoxic with 5% FCS in the media compared with controls, although cell survival was higher than with 2% FCS. The cytoprotective action of FCS was clearly shown at the concentration of 10% at which AgNO3 cytotoxicity of 7 x 10(-5) % to 28 x 10(-5) %, was partially or completely inhibited. Our results show that AgNO3 at concentrations 100-700 times more diluted than that normally used in clinical practice retained effective inhibitory activity against some of the above-mentioned microorganisms. However, even these concentrations are cytotoxic far cultured fibroblasts. Thus, silver nitrate concentrations up to 100 times more diluted can be used, since they possess bacterial growth-inhibiting power, are less cytotoxic and therefore favour wound healing.
引用
收藏
页码:140 / 151
页数:12
相关论文
共 35 条
[1]   Growth factor modulation of fibroblasts in simulated wound healing [J].
Bartold, PM ;
Raben, A .
JOURNAL OF PERIODONTAL RESEARCH, 1996, 31 (03) :205-216
[2]   TOXICITY DETERMINED INVITRO BY MORPHOLOGICAL ALTERATIONS AND NEUTRAL RED ABSORPTION [J].
BORENFREUND, E ;
PUERNER, JA .
TOXICOLOGY LETTERS, 1985, 24 (2-3) :119-124
[3]   NONCYTOTOXIC COMBINATIONS OF TOPICAL ANTIMICROBIAL AGENTS FOR USE WITH CULTURED SKIN SUBSTITUTES [J].
BOYCE, ST ;
WARDEN, GD ;
HOLDER, IA .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1324-1328
[4]   SELECTION OF TOPICAL ANTIMICROBIAL AGENTS FOR CULTURED SKIN FOR BURNS BY COMBINED ASSESSMENT OF CELLULAR CYTOTOXICITY AND ANTIMICROBIAL ACTIVITY [J].
BOYCE, ST ;
HOLDER, IA .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1993, 92 (03) :493-500
[5]  
CLOTHIER RH, 1995, ATLA-ALTERN LAB ANIM, V23, P75
[6]   THE CYTOTOXIC EFFECTS OF COMMONLY USED TOPICAL ANTIMICROBIAL AGENTS ON HUMAN FIBROBLASTS AND KERATINOCYTES [J].
COOPER, ML ;
LAXER, JA ;
HANSBROUGH, JF .
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, 1991, 31 (06) :775-784
[7]   CYTOTOXICITY EVALUATION OF ANTISEPTICS AND ANTIBIOTICS ON CULTURED HUMAN FIBROBLASTS AND KERATINOCYTES [J].
DAMOUR, O ;
HUA, SZ ;
LASNE, F ;
VILLAIN, M ;
ROUSSELLE, P ;
COLLOMBEL, C .
BURNS, 1992, 18 (06) :479-485
[8]   Cellular and biochemical aspects of normal and abnormal wound healing: An overview [J].
Diegelmann, RF .
JOURNAL OF UROLOGY, 1997, 157 (01) :298-302
[9]  
*DRUGD ED STAFF DR, 1997, DRUG EV MON SILV NIT, P91
[10]  
FABREGUETTE A, 1994, PATHOL BIOL, V42, P888