Higher-throughput screening for Caco-2 permeability utilizing a multiple sprayer liquid chromatography/tandem mass spectrometry system

被引:37
作者
Fung, EN [1 ]
Chu, IH [1 ]
Li, C [1 ]
Liu, TT [1 ]
Soares, A [1 ]
Morrison, R [1 ]
Nomeir, AA [1 ]
机构
[1] Schering Plough Res Inst, Dept Drug Metab & Pharmacokinet, Exploratory Drug Metab, Kenilworth, NJ 07033 USA
关键词
D O I
10.1002/rcm.1165
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In the current drug discovery environment, high er-through put analytical assays have become essential to keep pace with the screening demands for drug metabolism and pharmacokinetics (DMPK) attributes. This has been dictated by advances primarily in chemical procedures, notably combinatorial and parallel syntheses, which has resulted in many-fold increases in the number of compounds requiring DMPK evaluation. Because of its speed and specificity, liquid chromatography/tandem mass spectrometry (LC/MS/MS) has become the dominant technology for sample analysis in the DMPK screening assays. For higher-throughput assays, analytical speed as well as other factors such as method development, data processing, quality control, and report generation, must be optimized. The four-way multiplexed electrospray interface (MUX(R)), which allows for the analysis of four LC eluents simultaneously, has been adopted to maximize the rate of sample introduction into the mass spectrometer. Generic fast-gradient HPLC methods that are suitable for approximately 80% of the new chemical entities encountered have been developed. In-house-written software programs have been used to streamline information flow within the system, and for quality control by automatically identifying analytical anomalies. By integrating these components together with automated method development and data processing, a system capable of screening 100 compounds per week for Caco-2 permeability has been established. Copyright (C) 2003 John Wiley Sons, Ltd.
引用
收藏
页码:2147 / 2152
页数:6
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