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Exploring the genome of Trypanosoma vivax through GSS and in silico comparative analysis

被引：3
作者：
Guerreiro, LTA
Souza, SS
Wagner, G
De Souza, EA
Mendes, PN
Campos, LM
Barros, L
Pires, PF
Campos, MLM
Grisard, EC
Dávila, AMR
机构：
[1] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, Rio De Janeiro, Brazil
[2] Univ Fed Santa Catarina, Dept Microbiol & Parasitol, Florianopolis, SC, Brazil
[3] Predio Ctr Ciencias Matemat & Nat, Dept Ciencia Comp, Rio De Janeiro, Brazil
来源：
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY | 2005年 / 9卷 / 01期
关键词：
D O I：
10.1089/omi.2005.9.116
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A survey of the Trypanosoma vivax genome was carried out by the genome sequence survey (GSS) approach resulting in 1,086 genomic sequences. A total of 455 high-quality GSS sequences were generated, consisting of 331 non-redundant sequences distributed in 264 singlets and 67 clusters in a total of 135.5 Kb of the T. vivax genome. The estimation of the overall G + C content, and the prediction of the presence of ORFs and putative genes were carried out using the Glimmer and Jemboss packages. Analysis of the obtained sequences was carried out by BLAST programs against 12 different databases and also using the Conserved Domain Database, InterProScan, and tRNAscan-SE. Along with the existing 23 T. vivax entries in the GenBank, the 32 putative genes predicted and the 331 non-redundant GSS sequences reported herein represent new potential markers for the development of PCR-based assays for specific diagnosis and typing of Trypanosoma vivax.
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页码：116 / 128
页数：13
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