The construction of a substitution library of recombinant backcross lines in Brassica oleracea for the precision mapping of quantitative trait loci

被引：65

作者：

Ramsay, LD ^{[1
]}

Jennings, DE ^{[1
]}

Bohuon, EJR ^{[1
]}

Arthur, AE ^{[1
]}

Lydiate, DJ ^{[1
]}

Kearsey, MJ ^{[1
]}

Marshall, DF ^{[1
]}

机构：

[1] JOHN INNES CTR PLANT SCI RES,BRASSICA & OIL SEEDS RES DEPT,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

GENOME | 1996年 / 39卷 / 03期

关键词：

backcrossing; Brassica oleracea; introgression; molecular markers; near-isogenic lines; QTL mapping; recombinant backcross lines; substitution lines;

D O I：

10.1139/g96-071

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The currently available methods for locating quantitative trail loci (QTLs) and measuring their effects in segregating populations lack precision unless individual QTLs have very high heritabilities. The use of recombinant backcross lines containing short regions of donor chromosome introgressed into a constant recipient background permits QTLs to be located with greater precision. The present paper describes the use of molecular markers to introgress defined short regions of chromosome from a donor doubled haploid calabrese line of Brassica oleracea (var. italica) into a recipient short generation variety (Brassica oleracea var. alboglabra). We demonstrate that in just two or three generations of backcrossing, combined with selection for mapped molecular markers, the generation of a library of recombinant backcross lines is feasible. The possible use and refinement of these lines are discussed.

引用

页码：558 / 567

页数：10

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