Langmuir-Blodgett film of hydrogenase for electrochemical hydrogen production

被引：21

作者：

Noda, K

Zorin, NA

Nakamura, C

Miyake, M

Gogotov, IN

Asada, Y

Akutsu, H

Miyake, J

机构：

[1] AIST, MITI, Natl Inst Adv Interdisciplinary Res, Ibaraki 3058562, Japan

[2] Yokohama Natl Univ, Fac Engn, Yokohama, Kanagawa 2408501, Japan

[3] Russian Acad Sci, Inst Soil Sci & Photosynth, Pushchino 142292, Russia

[4] AIST, Natl Inst Biosci & Human Technol, Ibaraki 3058566, Japan

来源：

THIN SOLID FILMS | 1998年 / 327卷

基金：

俄罗斯基础研究基金会;

关键词：

Langmuir-Blodgett films; hydrogen; biomaterials; electrochemistry; hydrogenase;

D O I：

10.1016/S0040-6090(98)00730-5

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

LB films of thermostable hydrogenase from Thiocapsa roseopersicina were prepared on electrodes. Poly-L-lysine was used as a counter ion which stabilizes the protein and improves the protein transfer onto substrates. Both hydrogen production and oxidation activities were observed in the hydrogenase monolayer on substrate. The monolayer film of hydrogenase showed a high thermal stability as in solution, i.e. denaturation did not occur up to 70 degrees C. Electrochemical hydrogen evolution was observed by biasing the ITO electrode on which the LB film of hydrogenase was mounted in the presence of methyl viologen as a mediator. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：639 / 642

页数：4

共 14 条

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