Oxidative stress response in eukaryotes:: effect of glutathione, superoxide dismutase and catalase on adaptation to peroxide and menadione stresses in Saccharomyces cerevisiae

被引:47
作者
Fernandes, Patricia N. [1 ]
Mannarino, Sergio C. [1 ]
Silva, Carmelita G. [1 ]
Pereira, Marcos D. [1 ]
Panek, Anita D. [1 ]
Eleutherio, Elis C. A. [1 ]
机构
[1] Univ Fed Rio de Janeiro, Inst Quim, Dept Bioquim, BR-21941909 Rio de Janeiro, Brazil
基金
巴西圣保罗研究基金会;
关键词
peroxide; menadione; catalase; superoxide dismutase; glutathione; Saccharomyces cerevisiae;
D O I
10.1179/135100007X200344
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Aiming to clarify the mechanisms by which eukaryotes acquire tolerance to oxidative stress, adaptive and cross-protection responses to oxidants were investigated in Saccharomyces cerevisiae. Cells treated with sub-lethal concentrations of menadione (a source of superoxide anions) exhibited cross-protection against lethal doses of peroxide; however, cells treated with H2O2 did not acquire tolerance to a menadione stress, indicating that menadione response encompasses H2O2 adaptation. Although, deficiency in cytoplasmic superoxide dismutase (Sod1) had not interfered with response to superoxide, cells deficient in glutathione (GSH) synthesis were not able to acquire tolerance to H2O2 when pretreated with menadione. These results suggest that GSH is an inducible part of the superoxide adaptive stress response, which correlates with a decrease in the levels of intracellular oxidation. On the other hand, neither the deficiency of Sod1 nor in GSH impaired the process of acquisition of tolerance to H2O2 achieved by a mild pretreatment with peroxide. Using a strain deficient in the cytosolic catalase, we were able to conclude that the reduction in lipid peroxidation levels produced by the adaptive treatment with H2O2 was dependent on this enzyme. Corroborating these results, the pretreatment with low concentrations of H2O2 promoted an increase in catalase activity.
引用
收藏
页码:236 / 244
页数:9
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