Bile salts and glycine as cogerminants for Clostridium difficile spores

被引:525
作者
Sorg, Joseph A. [1 ]
Sonenshein, Abraham L. [1 ]
机构
[1] Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02111 USA
关键词
D O I
10.1128/JB.01765-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Spore formation by Clostridium difficile is a significant obstacle to overcoming hospital-acquired C difficile-associated disease. Spores are resistant to heat, radiation, chemicals, and antibiotics, making a contaminated environment difficult to clean. To cause disease, however, spores must germinate and grow out as vegetative cells. The germination of C difficile spores has not been examined in detail. In an effort to understand the germination of C difficile spores, we characterized the response of C difficile spores to bile. We found that cholate derivatives and the amino acid glycine act as cogerminants. Deoxycholate, a metabolite of cholate produced by the normal intestinal flora, also induced germination of C difficile spores but prevented the growth of vegetative C. difficile. A model of resistance to C difficile colonization mediated by the normal bacterial flora is proposed.
引用
收藏
页码:2505 / 2512
页数:8
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