Human cytomegalovirus IE1 protein activates AP-1 through a cellular protein kinase(s)

被引:25
作者
Kim, S [1 ]
Yu, SS
Lee, IS
Ohno, S
Yim, J
Kim, S [1 ]
Kang, HS
机构
[1] Yokohama City Univ, Sch Med, Dept Biol Mol, Yokohama, Kanagawa 236, Japan
[2] Seoul Natl Univ, Coll Nat Sci, Dept Microbiol, Seoul 151742, South Korea
[3] Seoul Natl Univ, Inst Mol Biol & Genet, Seoul 151742, South Korea
关键词
D O I
10.1099/0022-1317-80-4-961
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
During human cytomegalovirus (HCMV) infection, a rapid increase in AP-1 activity is detected. In this study, activation of transcription from promoters containing AP-1-binding sites by the IE1 protein of HCMV was examined. In transient transfection assays with reporter plasmids, it was found that IE1 strongly induced AP-l-driven transcription. Cells stably expressing IE1 also showed higher levels of AP-1 activity than did control cells. IE1 expression did not raise levels of c-jun and c-fos RNA, as determined by quantitative RT-PCR. AP-1 induction by IE1 was blocked efficiently by protein kinase inhibitors in a cell type-dependent manner; for example, by staurosporine in the human microglial cell line U373MG and by H7 in the human promonocytic cell line U937. IE1-driven activation of AP-1 was increased dramatically by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 1 (MEKK1). The results of this study indicate that IE1 activates AP-1 at the post-transcriptional level and that MEKK1 may play an important role in this process.
引用
收藏
页码:961 / 969
页数:9
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