Feulgen staining of intact plant tissues for confocal microscopy

被引：50

作者：

Braselton, JP ^{[1
]}

Wilkinson, MJ ^{[1
]}

Clulow, SA ^{[1
]}

机构：

[1] SCOTTISH CROP RES INST,CROP GENET DEPT,DUNDEE DD2 5DA,SCOTLAND

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1996年 / 71卷 / 02期

关键词：

chromosomes; confocal microscopy; fertilization; Feulgen; pararosaniline; Schiff;

D O I：

10.3109/10520299609117139

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method was developed to prepare plant structures for confocal laser scanning microscopy by combining Feulgen staining with pararosaniline and embedding in LR White(TM). This procedure preserves intact, delicate structures for three-dimensional imaging without loss from sectioning or squashing, and the slides can be viewed several times without serious photobleaching.

引用

页码：84 / 87

页数：4

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