Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay

被引:88
作者
Shi, YL
Yi, YP
Li, P
Kuang, TJ
Li, LH
Dong, M
Ma, QJ
Cao, C
机构
[1] Beijing Inst Biotechnol, Beijing 100850, Peoples R China
[2] Gen Hosp Guangzhou Command, Peoples Liberat Army, Guangzhou 510010, Peoples R China
[3] 309 army Hosp, Peoples Liberat Army, Beijing 100083, Peoples R China
关键词
D O I
10.1128/JCM.41.12.5781-5782.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (ELISA). Antinucleocapsid protein antibodies could be detected in 68.4% of probable SARS patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. No false-positive results were observed in 20 non-SARS fever patients, 24 non-SARS respiratory illness patients, and 20 health care workers. Among 940 other non-SARS clinical serum samples, only 1 was found to be weakly positive. This method provides a new, sensitive, and specific approach for SARS diagnosis.
引用
收藏
页码:5781 / 5782
页数:2
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