Melatonin receptor subtype expression in human cerebellum

被引:103
作者
Al-Ghoul, WM
Herman, MD
Dubocovich, ML
机构
[1] Northwestern Univ, Sch Med, Dept Biol Chem & Mol Pharmacol S215, Chicago, IL 60611 USA
[2] Northwestern Univ, Sch Med, Inst Neurosci, NW Drug Discovery Program, Chicago, IL 60611 USA
[3] Lutheran Gen Hosp, Dept Neurosurg, Park Ridge, IL 60068 USA
关键词
human cerebellum; 2-[I-125]iodomelatonin; Mel(1a); Mel(1b); Mel(1c); melatonin receptor subtypes; molecular and granular layers; mt(1) mRNA; MT2; mRNA; receptor autoradiography;
D O I
10.1097/00001756-199812210-00011
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
WE report the distinct cellular distribution in the human cerebellar cortex of the mammalian mt(1) (Mel(1a)) and MT2 (Mel(1b)) (1) melatonin receptor subtypes. Specific binding of the non-selective radioligand 2-[I-125]iodomelatonin to the outer molecular layer was significantly higher than to the granule cell layer. Melatonin receptor subtype expression was assessed by in situ hybridization using selective and specific digoxigenin-labeled antisense oligonucleotide probes. This is the first demonstration of MT2 melatonin receptor mRNA expression in human cerebellar Bergmann glia and astrocytes. On the other hand, the mt(1) melatonin receptor mRNA was expressed in both basket-stellate cells and granule cells. We conclude that mt(1) and MT2 melatonin receptors are heterogeneously expressed in human cerebellar cortex. (C) 1998 Lippincott Williams & Wilkins.
引用
收藏
页码:4063 / 4068
页数:6
相关论文
共 23 条
[1]   DIFFERENTIAL EXPRESSION OF 5 N-METHYL-D-ASPARTATE RECEPTOR SUBUNIT MESSENGER-RNAS IN THE CEREBELLUM OF DEVELOPING-RATS AND ADULT-RATS [J].
AKAZAWA, C ;
SHIGEMOTO, R ;
BESSHO, Y ;
NAKANISHI, S ;
MIZUNO, N .
JOURNAL OF COMPARATIVE NEUROLOGY, 1994, 347 (01) :150-160
[2]  
Beresford IJM, 1998, J PHARMACOL EXP THER, V285, P1239
[3]   Melatonin mediates two distinct responses in vascular smooth muscle [J].
Doolen, S ;
Krause, DN ;
Dubocovich, ML ;
Duckles, SP .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1998, 345 (01) :67-69
[4]   Melatonin receptor antagonists that differentiate between the human Mel(1a), and Mel(1b) recombinant subtypes are used to assess the pharmacological profile of the rabbit retina ML(1) presynaptic heteroreceptor [J].
Dubocovich, ML ;
Masana, MI ;
Iacob, S ;
Sauri, DM .
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1997, 355 (03) :365-375
[5]   Selective MT2 melatonin receptor antagonists block melatonin-mediated phase advances of circadian rhythms [J].
Dubocovich, ML ;
Yun, K ;
Al-Ghoul, WM ;
Benloucif, S ;
Masana, MI .
FASEB JOURNAL, 1998, 12 (12) :1211-1220
[6]   MELATONIN RECEPTORS - ARE THERE MULTIPLE SUBTYPES [J].
DUBOCOVICH, ML .
TRENDS IN PHARMACOLOGICAL SCIENCES, 1995, 16 (02) :50-56
[7]  
Dubocovich ML, 1998, IUPHAR COMPENDIUM RE, P187
[8]   The Mel(1a) melatonin receptor is coupled to parallel signal transduction pathways [J].
Godson, C ;
Reppert, SM .
ENDOCRINOLOGY, 1997, 138 (01) :397-404
[9]  
Hamadanizadeh S. A., 1997, Society for Neuroscience Abstracts, V23, P1492
[10]   GLUTAMATE-RECEPTOR SUBUNITS AT MOSSY FIBER-UNIPOLAR BRUSH CELL SYNAPSES - LIGHT AND ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMICAL STUDY IN CEREBELLAR CORTEX OF RAT AND CAT [J].
JAARSMA, D ;
WENTHOLD, RJ ;
MUGNAINI, E .
JOURNAL OF COMPARATIVE NEUROLOGY, 1995, 357 (01) :145-160