Killing effect and antitoxic activity of the Leptospira interrogans toxin-antitoxin system in Escherichia coli

被引：16

作者：

Picardeau, M

Ren, SX

Girons, IS

机构：

[1] Inst Pasteur, Unite Bacteriol Mol & Med, F-75724 Paris 15, France

[2] Chinese Human Genome Ctr, Shanghai, Peoples R China

来源：

JOURNAL OF BACTERIOLOGY | 2001年 / 183卷 / 21期

关键词：

D O I：

10.1128/JB.183.21.6494-6497.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the first evidence of a chromosome-encoded toxin-antitoxin locus in spirochetes. This locus has been found in the pathogenic spirochete Leptospira interrogans and exhibits homologies with the pem/chp loci. The L. interrogans chp locus consists of two genes: chpK (for "killer protein") and its upstream partner chpI (for "inhibitory protein"). Expression of ChpK in, Escherichia coli results in the inhibition of bacterial growth. The coexpression of ChpI neutralizes, ChpK toxicity By Southern blot analysis, chp homologs were found in all representative pathogenic strains of L. interrogans.

引用

页码：6494 / 6497

页数：4

共 10 条

[1] Addiction modules and programmed cell death and antideath in bacterial cultures [J].

Engelberg-Kulka, H ;

Glaser, G .

ANNUAL REVIEW OF MICROBIOLOGY, 1999, 53 :43-70

[2] WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD [J].

FLEISCHMANN, RD ;

ADAMS, MD ;

WHITE, O ;

CLAYTON, RA ;

KIRKNESS, EF ;

KERLAVAGE, AR ;

BULT, CJ ;

TOMB, JF ;

DOUGHERTY, BA ;

MERRICK, JM ;

MCKENNEY, K ;

SUTTON, G ;

FITZHUGH, W ;

FIELDS, C ;

GOCAYNE, JD ;

SCOTT, J ;

SHIRLEY, R ;

LIU, LI ;

GLODEK, A ;

KELLEY, JM ;

WEIDMAN, JF ;

PHILLIPS, CA ;

SPRIGGS, T ;

HEDBLOM, E ;

COTTON, MD ;

UTTERBACK, TR ;

HANNA, MC ;

NGUYEN, DT ;

SAUDEK, DM ;

BRANDON, RC ;

FINE, LD ;

FRITCHMAN, JL ;

FUHRMANN, JL ;

GEOGHAGEN, NSM ;

GNEHM, CL ;

MCDONALD, LA ;

SMALL, KV ;

FRASER, CM ;

SMITH, HO ;

VENTER, JC .

SCIENCE, 1995, 269 (5223) :496-512

[3] Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi [J].

Fraser, CM ;

Casjens, S ;

Huang, WM ;

Sutton, GG ;

Clayton, R ;

Lathigra, R ;

White, O ;

Ketchum, KA ;

Dodson, R ;

Hickey, EK ;

Gwinn, M ;

Dougherty, B ;

Tomb, JF ;

Fleischmann, RD ;

Richardson, D ;

Peterson, J ;

Kerlavage, AR ;

Quackenbush, J ;

Salzberg, S ;

Hanson, M ;

vanVugt, R ;

Palmer, N ;

Adams, MD ;

Gocayne, J ;

Weidman, J ;

Utterback, T ;

Watthey, L ;

McDonald, L ;

Artiach, P ;

Bowman, C ;

Garland, S ;

Fujii, C ;

Cotton, MD ;

Horst, K ;

Roberts, K ;

Hatch, B ;

Smith, HO ;

Venter, JC .

NATURE, 1997, 390 (6660) :580-586

[4] Complete genome sequence of Treponema pallidum, the syphilis spirochete [J].

Fraser, CM ;

Norris, SJ ;

Weinstock, CM ;

White, O ;

Sutton, GG ;

Dodson, R ;

Gwinn, M ;

Hickey, EK ;

Clayton, R ;

Ketchum, KA ;

Sodergren, E ;

Hardham, JM ;

McLeod, MP ;

Salzberg, S ;

Peterson, J ;

Khalak, H ;

Richardson, D ;

Howell, JK ;

Chidambaram, M ;

Utterback, T ;

McDonald, L ;

Artiach, P ;

Bowman, C ;

Cotton, MD ;

Fujii, C ;

Garland, S ;

Hatch, B ;

Horst, K ;

Roberts, K ;

Sandusky, M ;

Weidman, J ;

Smith, HO ;

Venter, JC .

SCIENCE, 1998, 281 (5375) :375-388

[5] Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress [J].

Gerdes, K .

JOURNAL OF BACTERIOLOGY, 2000, 182 (03) :561-572

[6] Bacterial toxin-antitoxin gene system as containment control in yeast cells [J].

Kristoffersen, P ;

Jensen, GB ;

Gerdes, K ;

Piskur, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (12) :5524-5526

[7] CHPA AND CHPB, ESCHERICHIA-COLI CHROMOSOMAL HOMOLOGS OF THE PEM LOCUS RESPONSIBLE FOR STABLE MAINTENANCE OF PLASMID R100 [J].

MASUDA, YJ ;

MIYAKAWA, K ;

NISHIMURA, Y ;

OHTSUBO, E .

JOURNAL OF BACTERIOLOGY, 1993, 175 (21) :6850-6856

[8] First evidence for gene replacement in Leptospira spp. Inactivation of L-biflexa flaB results in non-motile mutants deficient in endoflagella [J].

Picardeau, M ;

Brenot, A ;

Saint Girons, I .

MOLECULAR MICROBIOLOGY, 2001, 40 (01) :189-199

[9]

RUIZECHEVARRIA MJ, 1995, J MOL BIOL, V247, P568, DOI 10.1016/S0022-2836(05)80138-X

[10] AUTOREGULATION BY COOPERATIVE BINDING OF THE PEMI AND PEMK PROTEINS TO THE PROMOTER REGION OF THE PEM OPERON [J].

TSUCHIMOTO, S ;

OHTSUBO, E .

MOLECULAR & GENERAL GENETICS, 1993, 237 (1-2) :81-88

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