Protein-protein interactions with subunits of human nuclear RNase P

被引：53

作者：

Jiang, TJ ^{[1
]}

Altman, S ^{[1
]}

机构：

[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 03期

关键词：

heat shock; Hsp27; nuclear proteins; precursor tRNA;

D O I：

10.1073/pnas.021561498

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A yeast two-hybrid system was used to analyze interactions among the; protein subunits of human nuclear RNase P themselves and with other interacting partners encoded in a HeLa cell cDNA library. Subunits hpop1,Rpp21, Rpp29, Rpp30, Rpp38, and Rpp40 are involved in extensive, but weak, protein-protein interactions in the holoenzyme complex. Rpp14, Rpp20, and Rpp30 were found to have strong interactions with proteins encoded in the cDNA library. The small heat shock protein 27, which interacts with Rpp20 in the two-hybrid assay, binds to Rpp20 during affinity chromatography and can be found to be associated with, and enhances the activity of, highly purified RNase P, RNase P activity in HeLa cell nuclei also increases under the stress of heat shock.

引用

页码：920 / 925

页数：6

共 31 条

[1] The yeast exosome and human PM-Scl are related complexes of 3′→5′ exonucleases [J].

Allmang, C ;

Petfalski, E ;

Podtelejnikov, A ;

Mann, M ;

Tollervey, D ;

Mitchell, P .

GENES & DEVELOPMENT, 1999, 13 (16) :2148-2158

[2]

Altman S., 1999, Cold Spring Harbor Monograph Archive, V37, P351

[3] DYNAMIC CHANGES IN THE STRUCTURE AND INTRACELLULAR LOCALE OF THE MAMMALIAN LOW-MOLECULAR-WEIGHT HEAT-SHOCK PROTEIN [J].

ARRIGO, AP ;

SUHAN, JP ;

WELCH, WJ .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (12) :5059-5071

[4]

ARRIGO AP, 1994, HEAT SHOCK PROTEINS, P335

[5] HEAT-SHOCK BUT NOT OTHER STRESS INDUCERS LEADS TO THE DISRUPTION OF A SUBSET OF SNRNPS AND INHIBITION OF INVITRO SPLICING IN HELA-CELLS [J].

BOND, U .

EMBO JOURNAL, 1988, 7 (11) :3509-3518

[6] Hsp27 negatively regulates cell death by interacting with cytochrome c [J].

Bruey, JM ;

Ducasse, C ;

Bonniaud, P ;

Ravagnan, L ;

Susin, SA ;

Diaz-Latoud, C ;

Gurbuxani, S ;

Arrigo, AP ;

Kroemer, G ;

Solary, E ;

Garrido, C .

NATURE CELL BIOLOGY, 2000, 2 (09) :645-652

[7] Inhibition of Daxx-mediated apoptosis by heat shock protein 27 [J].

Charette, SJ ;

Lavoie, JN ;

Lambert, H ;

Landry, J .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (20) :7602-7612

[8] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[9]

Cuesta R, 2000, GENE DEV, V14, P1460

[10] Heat shock inhibits radiation-induced activation of NF-κB via inhibition of I-κB kinase [J].

Curry, HA ;

Clemens, RA ;

Shah, S ;

Bradbury, CM ;

Botero, A ;

Goswami, P ;

Gius, D .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (33) :23061-23067

← 1 2 3 4 →