ADAM23 methylation and expression analysis in brain tumors

被引：17

作者：

Costa, FF

Colin, C

Shinjo, SMO

Zanata, SM

Marie, SKN

Sogayar, MC

Camargo, AA ^{[1
]}

机构：

[1] Ludwig Inst Canc Res, Lab Mol Biol & Genom, Sao Paulo, Brazil

[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, Brazil

[3] Univ Sao Paulo, Fac Med, Dept Neurol, Sao Paulo, Brazil

[4] Univ Fed Parana, Dept Basic Pathol, Lab Neurobiol, Parana, SP, Brazil

来源：

NEUROSCIENCE LETTERS | 2005年 / 380卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

ADAM23; methylation; down-regulation and brain tumors;

D O I：

10.1016/j.neulet.2005.01.050

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

The ADAMS comprises a family of cell surface proteins with putative roles in cell-cell and/or cell-matrix interactions and in protease activities. In this work, we have examined the expression level and the methylation status of the 5' upstream region of the adhesion molecule ADAM23 in two brain tumor cell lines (A172 and T98G) as well as in three primary brain tumors (one grade II astrocytoma and two meningiomas) and 15 glioblastoma xenografts. Using bisulfite sequencing we verified that the percentage of methylated dinucleotides is higher in T98G when compared to A172 and that methylation significantly correlates with ADAM23 mRNA and protein expression. However, we were unable to detect methylation and down-regulation of the ADAM23 gene in brain tumors. Together, these results indicate that ADAM23 down-regulation by methylation in brain tumors is a rare event and it may help explain why brain tumor metastases are rarely found elsewhere in the body. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：260 / 264

页数：5

共 23 条

[1]

ADAM 23/MDC3, a human disintegrin that promotes cell adhesion via interaction with the αvβ3 integrin through an RGD-independent mechanism [J].