Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

被引：229

作者：

Baert, Leen ^{[1
]}

Wobus, Christiane E. ^{[3
]}

Van Coillie, Els ^{[2
]}

Thackray, Larissa B. ^{[3
]}

Debevere, Johan ^{[1
]}

Uyttendaele, Mieke ^{[1
]}

机构：

[1] Univ Ghent, Fac Biosci Engn, Dept Food Safety & Food Qual, Lab Food Microbiol & Food Preservat, B-9000 Ghent, Belgium

[2] Inst Agr & Fuisheries Res, Technol & Food Unit, B-9090 Melle, Belgium

[3] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 02期

关键词：

D O I：

10.1128/AEM.01039-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

引用

收藏

页码：543 / 546

页数：4

相关论文

共 24 条

[1] Conformational stability and disassembly of Norwalk virus-like particles - Effect of pH and temperature [J].

Ausar, Salvador F. ;

Foubert, Thomas R. ;

Hudson, Mary H. ;

Vedvick, Thomas S. ;

Middaugh, C. Russell .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (28) :19478-19488

[2] Virus-contaminated oysters: a three-month monitoring of oysters imported to Switzerland [J].

Beuret, C ;

Baumgartner, A ;

Schluep, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (04) :2292-2297

[3] Surrogates for the study of norovirus stability and inactivation in the environment:: A comparison of murine norovirus and feline calicivirus [J].

Cannon, Jennifer L. ;

Papafragkou, Efstathia ;

Park, Geunwoo W. ;

Osborne, Jason ;

Jaykus, Lee-Ann ;

Vinje, Jan .

JOURNAL OF FOOD PROTECTION, 2006, 69 (11) :2761-2765

[4] Real-time PCR quantification of human adenoviruses in urban rivers indicates genome prevalence but low infectivity [J].

Choi, S ;

Jiang, SC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (11) :7426-7433

[5] Laboratory efforts to cultivate noroviruses [J].

Duizer, E ;

Schwab, KJ ;

Neill, FH ;

Atmar, RL ;

Koopmans, MPG ;

Estes, MK .

JOURNAL OF GENERAL VIROLOGY, 2004, 85 :79-87

[6] POLIOVIRUS DETECTION IN WATER BY CELL-CULTURE AND NUCLEIC-ACID HYBRIDIZATION [J].

ENRIQUEZ, CE ;

ABBASZADEGAN, M ;

PEPPER, IL ;

RICHARDSON, KJ ;

GERBA, CP .

WATER RESEARCH, 1993, 27 (07) :1113-1118

[7] Presence of viral genomes in mineral water: A sufficient condition to assume infectious risk? [J].

Gassilloud, B ;

Schwartzbrod, L ;

Gantzer, C .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (07) :3965-3969

[8] Specific detection of Nipah virus using real-time RT-PCR (TaqMan) [J].

Guillaume, V ;

Lefeuvre, A ;

Faure, C ;

Marianneau, P ;

Buckland, R ;

Lam, SK ;

Wild, TF ;

Deubel, V .

JOURNAL OF VIROLOGICAL METHODS, 2004, 120 (02) :229-237

[9] Norwalk virus RNA is infectious in mammalian cells [J].

Guix, Susana ;

Asanaka, Miyuki ;

Katayama, Kazuhiko ;

Crawford, Sue E. ;

Neill, Frederick H. ;

Atmar, Robert L. ;

Estes, Mary K. .

JOURNAL OF VIROLOGY, 2007, 81 (22) :12238-12248

[10] Genetic analysis of the RNA polymerase gene of caliciviruses from dogs and cats [J].

Hashimoto, M ;

Roerink, F ;

Tohya, Y ;

Mochizuki, M .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 1999, 61 (06) :603-608