Partitioning of cortical and deep cytoskeleton responses from transient magnetic bead twisting

被引:57
作者
Laurent, VM [1 ]
Fodil, R [1 ]
Cañadas, P [1 ]
Féréol, S [1 ]
Louis, B [1 ]
Planus, E [1 ]
Isabey, D [1 ]
机构
[1] Univ Paris 12, Inst Super Biosci Paris, INSERM,UMR S 492, F-94010 Creteil, France
关键词
rheological model; F-actin; cellular mechanical properties; viscoelastic model; plasticity; micromanipulation; cell culture; spatial reconstructions;
D O I
10.1114/1.1616932
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We attempted to estimate in living adherent epithelial alveolar cells, the degree of structural and mechanical heterogeneity by considering two individualized cytoskeleton components, i.e., a submembranous "cortical" cytoskeleton and a "deep" cytoskeleton (CSK). F-actin structure characterizing each CSK component was visualized from spatial reconstructions at low and high density, respectively, especially in a 10-mum-cubic neighborhood including the bead. Specific mechanical properties (Young elastic and viscous modulus E and eta) were revealed after partitioning the magnetic twisting cytometry response using a double viscoelastic "solid" model with asymmetric plastic relaxation. Results show that the cortical CSK response is a faster (tau(1)less than or equal to0.7 s), softer (E-1: 63-109 Pa), moderately viscous (eta(1): 7-18 Pa s), slightly tensed, and easily damaged structure compared to the deep CSK structure which appears slower (tau(2)similar to 1/2 min), stiffer 2 (E-2: 95 - 204 Pa), highly viscous (eta(2): 760- 1967 Pa s), more tensed, and fully elastic, while exhibiting a larger stress hardening behavior. Adding drug depolymerizing actin filaments decreased predominantly the deep CSK stiffness. By contrast, an agent altering cell-matrix interactions affected essentially the cortical CSK stiffness. We concluded that partitioning the CSK within cortical and deep structures is largely consistent with their respective functional activities. (C) 2003 Biomedical Engineering Society.
引用
收藏
页码:1263 / 1278
页数:16
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