Optimizing conditions for tobacco BY-2 cell cycle synchronization - Rapid communication

被引：25

作者：

Samuels, AL ^{[1
]}

Meehl, J ^{[1
]}

Lipe, M ^{[1
]}

Staehelin, LA ^{[1
]}

机构：

[1] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA

来源：

PROTOPLASMA | 1998年 / 202卷 / 3-4期

关键词：

tobacco BY-2; cell cycle synchronization; aphidicolin; propyzamide;

D O I：

10.1007/BF01282551

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Tobacco BY-2 cells have become. a major tool in plant cell biological research, in part due to the availability of a cell cycle synchronization protocol. This method, pioneered by Nagata and coworkers, involves sequential treatments with aphidicolin (a DNA synthesis inhibitor) and propyzamide (a microtubule inhibitor which arrests mitosis). The effects of these inhibitors are reversible, allowing the cell culture to progress into M phase synchronously. However, attempts to reproduce high levels of synchrony with published protocols have not been uniformly successful. This paper describes critical parameters for cell cycle synchronization and documents the kinetics and variation typically found in using this protocol.

引用

页码：232 / 236

页数：5

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