The behaviour of the autonomous maize transposable element En/Spm in Arabidopsis thaliana allows efficient mutagenesis

被引:81
作者
Wisman, E [1 ]
Cardon, GH [1 ]
Fransz, P [1 ]
Saedler, H [1 ]
机构
[1] Max Planck Inst Zuchtungsforsch, D-50829 Koln, Germany
关键词
Arabidopsis thaliana; transposable element; En-1; mutagenesis; evolution;
D O I
10.1023/A:1006082009151
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The behavior of the autonomous maize transposable element En/Spm of maize was studied in Arabidopsis. Transgenic Arabidopsis plants carrying En-1 elements were propagated for 12 generations using a single seed descent procedure. The distribution and activity of the En-1 element was monitored using Southern DNA hybridisations in generations 1, 6 and 12. In the first generation the highest number of En-1 insertions per line was 7, which increased to 20 in generation 12. The average number of En-1 insertions increased only slightly in the population, due to a gradual accumulation of segregants that lost the transposable element. During the development of the En-1 mutagenised population the element remained active even in the high-copy lines. In situ hybridisation demonstrated that multiple En-1 insertions were distributed over all Arabidopsis chromosomes. From the initial En-1 mutagenised populations many unstable gene mutations were recovered, indicating that En-1 can be used as a efficient tool for gene tagging in Arabidopsis.
引用
收藏
页码:989 / 999
页数:11
相关论文
共 31 条
  • [1] TRANSPOSON TAGGING OF A MALE-STERILITY GENE IN ARABIDOPSIS
    AARTS, MGM
    DIRKSE, WG
    STIEKEMA, WJ
    PEREIRA, A
    [J]. NATURE, 1993, 363 (6431) : 715 - 717
  • [2] Molecular characterization of the CER1 gene of arabidopsis involved in epicuticular wax biosynthesis and pollen fertility
    Aarts, MGM
    Keijzer, CJ
    Stiekema, WJ
    Pereira, A
    [J]. PLANT CELL, 1995, 7 (12) : 2115 - 2127
  • [3] [Anonymous], 1989, MOBILE DNA-UK
  • [4] Use of Ac as an insertional mutagen in Arabidopsis
    Bhatt, AM
    Page, T
    Lawson, EJR
    Lister, C
    Dean, C
    [J]. PLANT JOURNAL, 1996, 9 (06) : 935 - 945
  • [5] THE TRANSPOSABLE ELEMENT TAM1 OF ANTIRRHINUM-MAJUS IS 17 KB LONG
    BONAS, U
    SOMMER, H
    HARRISON, BJ
    SAEDLER, H
    [J]. MOLECULAR AND GENERAL GENETICS, 1984, 194 (1-2): : 138 - 143
  • [6] COMPLEMENTARY FLORAL HOMEOTIC PHENOTYPES RESULT FROM OPPOSITE ORIENTATIONS OF A TRANSPOSON AT THE PLENA-LOCUS OF ANTIRRHINUM
    BRADLEY, D
    CARPENTER, R
    SOMMER, H
    HARTLEY, N
    COEN, E
    [J]. CELL, 1993, 72 (01) : 85 - 95
  • [7] SEQUENCE AND ORGANIZATION OF 5S RIBOSOMAL RNA-ENCODING GENES OF ARABIDOPSIS-THALIANA
    CAMPELL, BR
    SONG, YG
    POSCH, TE
    CULLIS, CA
    TOWN, CD
    [J]. GENE, 1992, 112 (02) : 225 - 228
  • [8] CARDON GH, 1993, PLANT J, V3, P773, DOI 10.1111/j.1365-313X.1993.00773.x
  • [9] COCCIOLONE SM, 1993, GENETICS, V135, P575
  • [10] THE DEFECTIVE EN-L102 ELEMENT ENCODES A PRODUCT REDUCING THE MUTABILITY OF THE EN SPM TRANSPOSABLE ELEMENT SYSTEM OF ZEA-MAYS
    CUYPERS, H
    DASH, S
    PETERSON, PA
    SAEDLER, H
    GIERL, A
    [J]. EMBO JOURNAL, 1988, 7 (10) : 2953 - 2960