Assessment of cytochrome P450 sequences offers a useful tool for determining genetic diversity in higher plant species

被引:35
作者
Yamanaka, S
Suzuki, E
Tanaka, M
Takeda, Y
Watanabe, JA
Watanabe, KN
机构
[1] Univ Tsukuba, Inst Biol Sci, Ctr Gene Res, Tsukuba, Ibaraki 3058572, Japan
[2] Kinki Univ, Fac Biol Oriented Sci & Technol, Wakayama 6496493, Japan
[3] Yokohama City Univ, Kihara Inst Biol Res, Yokohama, Kanagawa 2440813, Japan
[4] Nara Inst Sci & Technol, Ikoma 6300101, Japan
[5] Tokyo Rika Co Ltd, Tokyo, Japan
关键词
D O I
10.1007/s00122-003-1403-0
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
To investigate and develop new genetic tools for assessing genome-wide diversity in higher plant-species, polymorphisms of gene analogues of mammalian cytochrome P450 mono-oxygenases were studied. Data mining on Arabidopsis thaliana indicated that a small number of primer-sets derived from P450 genes could provide universal tools for the assessment of genome-wide genetic diversity in diverse plant species that do not have relevant genetic markers, or for which, there is no prior inheritance knowledge of inheritance traits. Results from PCR amplification of 51 plant species from 28 taxonomic families using P450 gene-primer sets suggested that there were at least several mammalian P450 gene mammalian-analogues in plants. Intra- and inter- specific variations were demonstrated following PCR amplifications of P450 analogue fragments, and this suggested that these would be effective genetic markers for the assessment of genetic diversity in plants. In addition, BLAST search analysis revealed that these amplified fragments possessed homologies to other genes and proteins in different plant varieties. We conclude that the sequence diversity of P450 gene-analogues in different plant species reflects the diversity of functional regions in the plant genome and is therefore an effective tool in functional genomic studies of plants.
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页码:1 / 9
页数:9
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