Platelet-rich fibrin modulates the expression of extracellular signal-regulated protein kinase and osteoprotegerin in human osteoblasts

被引：66

作者：

Chang, I-Chang ^{[4
,5
]}

Tsai, Chung-Hung ^{[3
]}

Chang, Yu-Chao ^{[1
,2
]}

机构：

[1] Chung Shan Med Univ Hosp, Dept Dent, Taichung, Taiwan

[2] Chung Shan Med Univ, Sch Dent, Taichung, Taiwan

[3] Chung Shan Med Univ Hosp, Dept Pathol, Taichung, Taiwan

[4] Chung Shan Med Univ Hosp, Dept Orthoped, Taichung, Taiwan

[5] Chung Shan Med Univ, Sch Med, Taichung, Taiwan

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2010年 / 95A卷 / 01期

关键词：

Choukroun's technique; platelet-rich fibrin; osteoblast; proliferation; osteoprotegerin; OSTEOCLASTOGENESIS-INHIBITORY FACTOR; B LIGAND EXPRESSION; GRAFTING MATERIAL; UP-REGULATION; GROWTH; PROLIFERATION; PRF; DIFFERENTIATION; CELLS; METRONIDAZOLE;

D O I：

10.1002/jbm.a.32839

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Platelet-rich fibrin (PRF) by Choukroun's technique is produced in a totally natural manner, without using anticoagulant during blood harvest nor bovine thrombin and calcium chloride for platelet activation and fibrin polymerization. When delicately pressed between two gauzes, the PRF clot becomes a strong membrane with high potential in clinical application and tissue engineering. In this study, blood collection was carried out from healthy volunteers. Osteoblast cell line U2OS was used to evaluate the cell proliferation resulting from PRF by using colorimetric assay. Western blot was employed to evaluate the expression of phosphorylated extracellular signal-regulated protein kinase (p-ERK), receptor activator of nuclear factor-kappa B ligand (RANKL), and osteoprotegerin (OPG) in U2OS cells. PRF was found to increase osteoblast proliferation during 5 day incubation period (p < 0.05). PRF was found to increase ERK phosphorylation in U2OS cells (p < 0.05). OPG was significantly elevated by the stimulation with PRF (p < 0.05). However, there was no significant change in RANKL expression (p > 0.05). Taken together, PRF can stimulate osteoblasts proliferation. The activation of p-ERK and OPG expression by PRF suggests a potential role for new bone formation. The application of PRF may provide the benefit for the bone regeneration. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 327-332, 2010.

引用

页码：327 / 332

页数：6

共 32 条

[1]

Braccini F, 2007, Rev Laryngol Otol Rhinol (Bord), V128, P255

[2]

The upregulation of receptor activator NF-κB ligand expression by interleukin-1α and Porphyromonas endodontalis in human osteoblastic cells [J].

Chen, S. -C. ;

Huang, F. -M. ;

Lee, S. -S. ;

Li, M. -Z. ;

Chang, Y. -C. .

INTERNATIONAL ENDODONTIC JOURNAL, 2009, 42 (04) :375-380

[3]

Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift [J].

Choukroun, J ;

Diss, A ;

Simonpieri, A ;

Girard, MO ;

Schoeffler, C ;

Dohan, SL ;

Dohan, AJJ ;

Mouhyi, J ;

Dohan, DM .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2006, 101 (03) :299-303

[4]

Choukroun J., 2000, IMPLANTODONTIE, V42, P55

[5]

Choukroun J I, 2007, Rev Laryngol Otol Rhinol (Bord), V128, P27

[6]

Osteotome sinus floor elevation using Choukroun's platelet-rich fibrin as grafting material: A 1-year prospective pilot study with microthreaded implants [J].

Diss, Antoine ;

Dohan, David M. ;

Mouhyi, Jaafar ;

Mahler, Patrick .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2008, 105 (05) :572-579

[7]

Dohan David M, 2006, Oral Surg Oral Med Oral Pathol Oral Radiol Endod, V101, pe51, DOI 10.1016/j.tripleo.2005.07.010

[8]

Dohan David M, 2006, Oral Surg Oral Med Oral Pathol Oral Radiol Endod, V101, pe45, DOI 10.1016/j.tripleo.2005.07.009

[9]

In vitro effects of Choukroun's PRF (platelet-rich fibrin) on human gingival fibroblasts, dermal prekeratinocytes, preadipocytes, and maxillofacial osteoblasts in primary cultures [J].

Ehrenfest, David M. Dohan ;

Diss, Antoine ;

Odin, Guillaume ;

Doglioli, Pierre ;

Hippolyte, Marie-Pascale ;

Charrier, Jean-Baptiste .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2009, 108 (03) :341-352

[10]

Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF) [J].

Ehrenfest, David M. Dohan ;

Rasmusson, Lars ;

Albrektsson, Tomas .

TRENDS IN BIOTECHNOLOGY, 2009, 27 (03) :158-167

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