Expanding the genetic code of Escherichia coli

被引：1195

作者：

Wang, L

Brock, A

Herberich, B

Schultz, PG

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[3] Novartis Res Fdn, Genom Inst, San Diego, CA 92121 USA

来源：

SCIENCE | 2001年 / 292卷 / 5516期

关键词：

D O I：

10.1126/science.1060077

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A unique transfer RNA (tRNA)/aminoacyl-tRNA synthetase pair has been generated that expands the number of genetically encoded amino acids in Escherichia coli, When introduced into E, coli, this pair leads to the in vivo incorporation of the synthetic amino acid O-methyl-L-tyrosine into protein in response to an amber nonsense codon, The fidelity of translation is greater than 99%, as determined by analysis of dihydrofolate reductase containing the unnatural amino acid. This approach should provide a general method for increasing the genetic repertoire of Living cells to include a variety of amino acids with novel structural, chemical, and physical properties not found in the common 20 amino acids.

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收藏

页码：498 / 500

页数：3

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