Frequency of clonal B lymphocytes in chronic myelogenous leukemia evaluated by fluorescence in situ hybridization

被引:9
作者
Al-Amin, A
Lennartz, K
Runde, V
Seeber, S
Opalka, B
Kloke, O
机构
[1] Univ Essen, Sch Med, Dept Internal Med, W German Canc Ctr, D-45122 Essen, Germany
[2] Univ Essen, Sch Med, Dept Cell Biol, W German Canc Ctr, D-45122 Essen, Germany
[3] Univ Essen, Sch Med, Dept Bone Marrow Transplant, W German Canc Ctr, D-45122 Essen, Germany
关键词
D O I
10.1016/S0165-4608(97)00435-4
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The demonstration of the Philadelphia (Ph) chromosome in B lymphocytes from patients with chronic myelogenous leukemia (CML) has provided evidence that the disorder originates in a pluripotent progenitor cell. Divergent results, however, exist as to the degree of contribution of clonally derived cells to the B-cell compartment. To address this issue, B lymphocytes were selected from the blood of seven patients in the chronic phase of Ph-positive CML and were examined with dual-color-fluoresence in situ hybridization for the presence of the Ph translocation. The purity of the B-cell preparations ranged from 88% to 97% (mean 93%). The Ph translocation was detected in 22-34% (mean, 27%) of the sorted B cells. There was no evidence that the duration of the disease affects the ratio of Ph positive and -negative B cells. In summary, clonally derived circulating B lymphocytes were present in all patients studied but made only minor contribution to this compartment. (C) Elsevier Science Inc., 1998.
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页码:45 / 47
页数:3
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