Dendrimer interactions with hydrophobic fluorescent probes and human serum albumin

被引：67

作者：

Shcharbin, D

Klajnert, B

Mazhul, V

Bryszewska, M

机构：

[1] Univ Lodz, Dept Gen Biophys, PL-90237 Lodz, Poland

[2] Byelarussian Acad Sci, Inst Photobiol, Minsk 220072, BELARUS

来源：

JOURNAL OF FLUORESCENCE | 2005年 / 15卷 / 01期

关键词：

PAMAM dendrimers; ANS; fluorescent probe; binding;

D O I：

10.1007/s10895-005-0209-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The interactions between three types of polyamidoamine dendrimers (with anionic, cationic, and neutral charge on a surface) and fluorescent dye 1-anilinonaphthalene-8-sulfonate (ANS) were studied. Double fluorimetric titration method was employed to estimate a binding constant and the number of binding centers. As fluorescent probes can serve as models of toxin molecules, dendrimers, and human serum albumin (HSA) abilities to bind ANS were compared. In the presence of HSA and dendrimers, ANS located both in HSA and in dendrimers, but the interactions between ANS and HSA were stronger.

引用

页码：21 / 28

页数：8

共 68 条

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