A four-base paired genetic helix with expanded size

被引：210

作者：

Liu, HB ^{[1
]}

Gao, JM ^{[1
]}

Lynch, SR ^{[1
]}

Saito, YD ^{[1
]}

Maynard, L ^{[1
]}

Kool, ET ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

SCIENCE | 2003年 / 302卷 / 5646期

关键词：

D O I：

10.1126/science.1088334

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a new molecular class of genetic-pairing system that has a native DNA backbone but has all four base pairs replaced by new, larger pairs. The base pairs include size-expanded analogs of thymine and of adenine, both extended by the width of a benzene ring (2.4 Angstrom). The expanded-diameter double helices are more thermodynamically stable than the Watson-Crick helix, likely because of enhanced base stacking. Structural data confirm a right-handed, double-stranded, and base-paired helical form. Because of the larger base size, all the pairs of this helical system are fluorescent, which suggests practical applications in detection of natural DNA and RNA. Our findings establish that there is no apparent structural or thermodynamic prohibition against genetic systems having sizes different from the natural one.

引用

页码：868 / 871

页数：4

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