Rapid removal of unincorporated label and proteins from DNA sequencing reactions

被引：6

作者：

Kaczorowski, T ^{[1
]}

Sektas, M ^{[1
]}

机构：

[1] UNIV WISCONSIN,MCARDLE LAB CANC RES,MADISON,WI 53706

来源：

MOLECULAR BIOTECHNOLOGY | 1996年 / 5卷 / 02期

关键词：

radiolabeling; sequenase; 2.0; SPEL-6; wizard resin;

D O I：

10.1007/BF02789066

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article presents a simple and rapid method for removal of unincorporated label and proteins from DNA sequencing reactions by using Wizard purification resin. This method can be successfully applied for preparation of end-labeled oligonucleotides free of unincorporated label, which is important in experiments (including DNA sequencing) when the level of background should be as low as possible. Also, this method is effective in removal of proteins from DNA sequencing reactions.

引用

页码：177 / 180

页数：4