Accelerating high quality bioanalytical LC/MS/MS assays using fused-core columns

被引：24

作者：

Badman, Ethan R. ^{[1
]}

Beardsley, Richard L. ^{[1
]}

Liang, Zhenmin ^{[1
]}

Bansal, Surendra ^{[1
]}

机构：

[1] Hoffmann La Roche Inc, Nonclin Safety, Nutley, NJ 07110 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 25期

关键词：

High throughput; Bioanalysis; LC/MS/MS; Quantitation; Fused-core column; CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY; THROUGHPUT QUANTITATIVE-ANALYSIS; LIQUID-CHROMATOGRAPHY; SMALL MOLECULES; SAMPLE PREPARATION; HUMAN PLASMA; RAT PLASMA; QUANTIFICATION; SEPARATION; DISCOVERY;

D O I：

10.1016/j.jchromb.2010.06.032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High quality, ultra-fast bioanalytical LC/MS/MS methods were developed using short columns packed with fused-core particles and high (1.0-3.0 mL/min) flow rates. For more than two years, at flow rates up to 3.0 mL/min, using 0.33 min non-ballistic gradients, these methods were shown to provide comparable or better performance than slower assays for accuracy, precision, sensitivity, specificity, and ruggedness, and met all criteria required by the bioanalytical regulatory guidance. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：2307 / 2313

页数：7

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