Focus on function:: Single molecule RNA enzymology

被引：33

作者：

Ditzler, Mark A.

Aleman, Elvin A.

Rueda, David ^{[1
]}

Walter, Nils G.

机构：

[1] Univ Michigan, Div Biophys Res, Single Mol Analysis Grp, Ann Arbor, MI 48109 USA

[2] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA

[3] Univ Michigan, Dept Chem, Single Mol Analysis Grp, Ann Arbor, MI 48109 USA

来源：

BIOPOLYMERS | 2007年 / 87卷 / 5-6期

关键词：

single molecule microscopy; fluorescence resonance energy transfer; ribozyme; ribosome; catalytic RNA;

D O I：

10.1002/bip.20819

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of RNA to catalyze chemical reactions was first demonstrated 25 years ago with the discovery that group I introns and RNase P function as RNA enzymes (ribozymes). Several additional ribozymes were subsequently identified, most notably the ribosome, followed by intense: mechanistic studies. More recently, the introduction of single molecule tools has dissected the kinetic steps of several ribozymes in unprecedented detail and has revealed surprising heterogeneity not evident from ensemble approaches. Still, many fundamental questions of how RNA enzymes work at the molecular level remain unanswered. This review surveys the current status of our understanding of RNA catalysis at the single molecule level and discusses the existing challenges and opportunities in developing suitable assays. (C) 2007 Wiley Periodicals, Inc.

引用

页码：302 / 316

页数：15

共 103 条

[1] Conformational heterogeneity at position U37 of an all-RNA hairpin ribozyme with implications for metal binding and the catalytic structure of the S-turn [J].