A comparison between osteogenic differentiation of human unrestricted somatic stem cells and mesenchymal stem cells from bone marrow and adipose tissue

被引：132

作者：

Shafiee, Abbas ^{[2
]}

Seyedjafari, Ehsan ^{[2
,3
]}

Soleimani, Masoud ^{[1
]}

Ahmadbeigi, Naser ^{[2
]}

Dinarvand, Peyman ^{[2
,4
]}

Ghaemi, Nasser ^{[3
]}

机构：

[1] Tarbiat Modares Univ, Dept Hematol, Fac Med Sci, Tehran 14115111, Iran

[2] Stem Cell Technol Res Ctr, Stem Cell Biol Dept, Tehran, Iran

[3] Univ Tehran, Dept Biotechnol, Coll Sci, Tehran, Iran

[4] Univ Tehran Med Sci, Fac Med, Tehran, Iran

来源：

BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 06期

关键词：

Human stem cells; Osteogenic differentiation; Tissue engineering; CORD BLOOD; COLLAGEN-SYNTHESIS; STROMAL CELLS; GLUCOCORTICOIDS; THERAPY; REPAIR;

D O I：

10.1007/s10529-011-0541-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

To evaluate the potential of three stem cells for cell therapy and tissue engineering applications, the biological behavior and osteogenic capacity of the newly introduced cord-blood-derived, unrestricted somatic stem cells (USSC) were compared with those of mesenchymal stem cells isolated from bone marrow (BM-MSC) and adipose tissue (AT-MSC). There was no significant difference between the rates of proliferation of the three stem cells. During osteogenic differentiation, alkaline phosphatase (ALP) activity peaked on day 7 in USSC compared to BM-MSC which showed the maximum value of ALP activity on day 14. However, BM-MSC had the highest ALP activity and mineralization during osteogenic induction. In addition, AT-MSC showed the lowest capacity for mineralization during differentiation and had the lowest ALP activity on days 7 and 14. Although AT-MSC expressed higher levels of collagen type I, osteonectin and BMP-2 in undifferentiated state, but these genes were expressed higher in BM-MSC during differentiation. BM-MSC also expressed higher levels of ALP, osteocalcin and Runx2 during induction. Taking together, BM-MSC showed the highest capacity for osteogenic differentiation and hold promising potential for bone tissue engineering and cell therapy applications.

引用

页码：1257 / 1264

页数：8

共 33 条

[1]

Secondary repair of alveolar clefts using human mesenchymal stem cells [J].

Behnia, Hossein ;

Khojasteh, Arash ;

Soleimani, Masoud ;

Tehranchi, Azita ;

Khoshzaban, Ahad ;

Keshel, Saeed Hidari ;

Atashi, Reza .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2009, 108 (02) :E1-E6

[2]

Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice [J].

Bhatia, M ;

Wang, JCY ;

Kapp, U ;

Bonnet, D ;

Dick, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (10) :5320-5325

[3]

The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects [J].

Bruder, SP ;

Kraus, KH ;

Goldberg, VM ;

Kadiyala, S .

JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1998, 80A (07) :985-996

[4]

EFFECT OF GLUCOCORTICOIDS ON TYPE-I COLLAGEN-SYNTHESIS, ALKALINE-PHOSPHATASE ACTIVITY, AND DEOXYRIBONUCLEIC-ACID CONTENT IN CULTURED RAT CALVARIAE [J].

CANALIS, E .

ENDOCRINOLOGY, 1983, 112 (03) :931-939

[5]

A tissue engineering approach to bone repair in large animal models and in clinical practice [J].

Cancedda, Ranieri ;

Giannoni, Paolo ;

Mastrogiacomo, Maddalena .

BIOMATERIALS, 2007, 28 (29) :4240-4250

[6]

Mesenchymal stem cells: Cell-based reconstructive therapy in orthopedics [J].

Caplan, AI .

TISSUE ENGINEERING, 2005, 11 (7-8) :1198-1211

[7]

Co-culture of bone marrow fibroblasts and endothelial cells on modified polycaprolactone substrates for enhanced potentials in bone tissue engineering [J].

Choong, Cleo S. N. ;

Hutmacher, Dietmar W. ;

Triffitt, James T. .

TISSUE ENGINEERING, 2006, 12 (09) :2521-2531

[8]

CORTISOL DOWN-REGULATES OSTEOBLAST ALPHA-1(I) PROCOLLAGEN MESSENGER-RNA BY TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL MECHANISMS [J].

DELANY, AM ;

GABBITAS, BY ;

CANALIS, E .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1995, 57 (03) :488-494

[9]

Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].

Dominici, M. ;

Le Blanc, K. ;

Mueller, I. ;

Slaper-Cortenbach, I. ;

Marini, F. C. ;

Krause, D. S. ;

Deans, R. J. ;

Keating, A. ;

Prockop, D. J. ;

Horwitz, E. M. .

CYTOTHERAPY, 2006, 8 (04) :315-317

[10]

Downregulation of extracellular matrix-related gene clusters during osteogenic differentiation of human bone marrow and adipose tissue-derived stromal cells [J].

Egusa, Hiroshi ;

Iida, Keisuke ;

Kobayashi, Munemasa ;

Lin, Terry Y. ;

Zhu, Min ;

Zuk, Patricia A. ;

Wang, Chiachien Jake ;

Thakor, Devang K. ;

Hedrick, Marc H. ;

Nishimura, Ichiro .

TISSUE ENGINEERING, 2007, 13 (10) :2589-2600

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