Direct observation of tiers in the energy landscape of a chromoprotein:: A single-molecule study

被引：131

作者：

Hofmann, C

Aartsma, TJ

Michel, H

Köhler, J

机构：

[1] Univ Bayreuth, Bayreuther Inst Makromol Forsch, D-95440 Bayreuth, Germany

[2] Leiden Univ, Dept Biophys, Huygens Lab, NL-2300 RA Leiden, Netherlands

[3] Max Planck Inst Biophys, Dept Mol Membrane Biol, D-60439 Frankfurt, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2003年 / 100卷 / 26期

关键词：

D O I：

10.1073/pnas.2533896100

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Single-molecule spectroscopic techniques were applied to individual pigments embedded in a chromoprotein. A sensitive tool to monitor structural fluctuations of the protein backbone in the local environment of the chromophore is provided by recording the changes of the spectral positions of the pigment absorptions as a function of time. The data provide information about the organization of the energy landscape of the protein in tiers that can be characterized by an average barrier height. Additionally, a correlation between the average barrier height within a distinct tier and the time scale of the structural fluctuations is observed.

引用

页码：15534 / 15538

页数：5

共 32 条

[1] DYNAMICS OF LIGAND-BINDING TO MYOGLOBIN [J].