Elevated plasma endothelial microparticles in preeclampsia

OBJECTIVE: The purpose of this study was to assess endothelial dysfunction in women with preeclampsia by measuring endothelial microparticles. STUDY DESIGN: A case-control study was conducted on 20 women with preeclampsia and 20 healthy pregnant women as control subjects. Endothelial microparticles were measured by flow cytometry with anti-CD31 and anti-CD42. CD31+/CD42+ platelet microparticles were also quantified. RESULTS: Plasma endothelial microparticles levels were elevated significantly in women with preeclampsia as compared with control subjects (mean SD and median [range]: 14,723 7,724 counts/mL and 12,378 counts/mL [1,442-33,772 counts/mL] vs 8406 2832 counts/mL and 9016 counts/ml- [3,381-12,806 counts/mL]; P < .001). Plasma platelet microparticles levels were not different among cases compared to control subjects (10,751 6,114 counts/mL and 9463 counts/mL [3,000-23,895 counts/mL] vs 7871 4344 counts/mL and 6462 counts/mL [444-18,947 counts/mL]; P =.208). No significant correlation was found between plasma endothelial microparticles and mean arterial pressure in cases or control subjects. CONCLUSION: The elevation of endothelial microparticles in women with preeclampsia supports the endothelial injury theory in preeclampsia.