Twelve new microsatellite markers for gilted seabream (Sparus aurata L.):: characterization, polymorphism and linkage

被引:40
作者
Launey, S [1 ]
Krieg, F
Haffray, P
Bruant, JS
Vanniers, A
Guyomard, R
机构
[1] INRA, Lab Genet Poissons, F-78352 Jouy En Josas, France
[2] INRA, SCRIBE, SYSAAF, F-35042 Rennes, France
[3] Ferme Marine Douhet, F-17840 La Bree Les Bains, France
[4] LABOGENA, F-78350 Jouy En Josas, France
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 03期
关键词
genetic conservation; genetic diversity management; microsatellites; Sparus aurata;
D O I
10.1046/j.1471-8286.2003.00482.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Twelve new microsatellite markers were isolated from the gilted seabream Sparus aurata L., a seawater fish distributed throughout the Mediterranean Sea and on the East Atlantic Coast and exploited in both fisheries and aquaculture. Characterization in 16 individuals from a hatchery population from France reveals three to 17 alleles and a mean expected heterozygosity of 0.752. Inheritance and linkage were assessed in a bi-parental cross, and using polymorphism and linkage information, two PCR-multiplex were developed for routine analysis of Sparus aurata populations.
引用
收藏
页码:457 / 459
页数:3
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