Culture of human mast cells from peripheral blood progenitors

被引：52

作者：

Saito, Hirohisa

Kato, Atsushi

Matsumoto, Kenji

Okayama, Yoshimichi

机构：

[1] Natl Res Inst Child Hlth & Dev, Dept Allergy & Immunol, Setagaya Ku, Tokyo 1578535, Japan

[2] RIKEN, Res Ctr Allergy & Immunol, Res Unit Allergy Transcriptome, Yokohama, Kanagawa, Japan

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 04期

关键词：

D O I：

10.1038/nprot.2006.344

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 10(4)-10(5) cells are retrieved in the CD34(+) fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 10(3)-10(4) mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 10(6)-10(7).

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收藏

页码：2178 / 2183

页数：6

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