Structure of epiglucan, a highly side-chain/branched (1→3;1→6)-β-glucan from the micro fungus Epicoccum nigrum Ehrenb. ex Schlecht

被引：52

作者：

Schmid, F

Stone, BA

McDougall, BM

Bacic, A

Martin, KL

Brownlee, RTC

Chai, E

Seviour, RJ ^{[1
]}

机构：

[1] La Trobe Univ, Biotechnol Res Ctr, Bendigo, Vic 3550, Australia

[2] La Trobe Univ, Sch Biochem, Bundoora, Vic 3083, Australia

[3] Univ Melbourne, Sch Bot, Plant Cell Biol Res Ctr, Melbourne, Vic 3010, Australia

[4] La Trobe Univ, Dept Chem, Bundoora, Vic 3083, Australia

[5] Univ Melbourne, Dept Chem Engn, CRC Ind Plant Biopolymers, Melbourne, Vic 3010, Australia

来源：

CARBOHYDRATE RESEARCH | 2001年 / 331卷 / 02期

关键词：

extracellular polysaccharide; (1 -> 3 : 1 -> 6)-beta-glucan; Epicoccum nigrum;

D O I：

10.1016/S0008-6215(01)00023-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The extracellular fungal polysaccharide, epiglucan, synthesised by Epicoccum nigrum is a side-chain/branched (1-->3:1 --> 6)-D-beta -glucan. Methylation analysis, C-13 DEFT NMR and specific enzymic digestion data show slight variation in branching frequency among the epiglucans from the three strains examined. The (1-->3)-beta -linked backbone has (1 --> 6)-beta -linked branches at frequencies greater than the homologous glucans, scleroglucan and schizophyllan, from Sclerotium spp. and Schizophyllum commune, respectively. The structural analyses do not allow a distinction to be made between structures I and II. [GRAPHICS] Epiglucan displays non-Newtonian shear thinning rheological properties, typical of these glucans. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：163 / 171

页数：9

共 33 条

[1] NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].