Sugar-regulated control of α-tubulin in maize cell suspension culture

被引:7
作者
Datta, R
Chourey, PS
机构
[1] Univ Florida, USDA ARS, Dept Plant Pathol, Gainesville, FL 32611 USA
[2] Univ Florida, Program Plant Mol & Cellular Biol, Gainesville, FL 32611 USA
关键词
alpha-tubulin; sugar-regulation; cell suspension culture; Zea mays;
D O I
10.1007/s002990000310
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The data reported here shows that alpha -tubulin, the subunit of microtubulin in the cytoskeleton, is regulated by sugars. A 48-h sugar-depletion treatment in EMS maize cell suspension culture medium led to a marked reduction in the levels of alpha -tubulin mRNA and protein. This response was reversible; the addition of metabolizable sugars led to a rapid increase in the levels of the alpha -tubulin transcript. Levels of alpha -tubulin protein also increased, albeit gradually. Surprisingly, there was little or no effect of phosphate depletion and phosphate addition on alpha -tubulin expression. Furthermore, the data show that sugars modulate a co-regulation of alpha -tubulin and cell-wall invertase. We propose that microtubules might be a major component of a sugar-signaling pathway that is regulated by, among other factors, cellular carbon metabolism.
引用
收藏
页码:262 / 266
页数:5
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