Acute effects of insulin on the activity of mitochondrial GPAT1 in primary adipocytes

被引：15

作者：

Bronnikov, Gennady E. ^{[1
,2
]}

Aboulaich, Nabila ^{[1
,2
]}

Vener, Alexander V. ^{[1
,2
]}

Stralfors, Peter ^{[1
,2
]}

机构：

[1] Linkoping Univ, Dept Clin & Expt Med, Div Cell Biol, SE-58185 Linkoping, Sweden

[2] Linkoping Univ, Diabet Res Ctr, SE-58185 Linkoping, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 367卷 / 01期

关键词：

insulin control; triacylglycerol; obesity; kinetics; phosphorylation; mass spectrometry;

D O I：

10.1016/j.bbrc.2007.12.127

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitochondrial enzyme 1-acyl-sn-glycerol-3-phosphate acyltransferase (mtGPAT1) catalyzes a rate-limiting step in triacylglycerol and glycerophospholipid biosynthesis, which can be modulated by protein kinases in cell free analyses. We report that treatment of primary rat adipocytes with insulin acutely affects the activity of mtGPAT1 by increasing V-MAX and K-M for the substrates glycerol-3-phosphate and palmitoyl-CoA. Proteolytic cleavage of isolated mitochondrial membranes and mass spectrometric peptide sequencing identify in vivo phosphorylation of serine 632 and serine 639 in mtGPAT1. These phosphorylation sites correspond to casein kinase-2 consensus sequences and are highly conserved in chordate animal, but not fly, fungal or plant, mtGPAT1. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：201 / 207

页数：7

共 30 条

[1] Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes [J].