Effect of a Combined Treatment With Growth Hormone and Melatonin in the Cardiological Aging on Male SAMP8 Mice

被引：41

作者：

Forman, Katherine ^{[1
]}

Vara, Elena ^{[2
]}

Garcia, Cruz ^{[2
]}

Kireev, Roman ^{[1
]}

Cuesta, Sara ^{[1
]}

Escames, Germaine ^{[3
]}

Tresguerres, J. A. F. ^{[1
]}

机构：

[1] Univ Complutense Madrid, Sch Med, Dept Physiol, E-28040 Madrid, Spain

[2] Univ Complutense Madrid, Sch Med, Dept Biochem & Mol Biol, E-28040 Madrid, Spain

[3] Univ Granada, Ctr Biomed Invest, Inst Biotechnol, E-18071 Granada, Spain

来源：

JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES | 2011年 / 66卷 / 08期

关键词：

Aging; Heart; Apoptosis; Inflammation; Oxidative stress; SENESCENCE-ACCELERATED MICE; NF-KAPPA-B; INFLAMMATORY GENE-EXPRESSION; OXIDATIVE STRESS PARAMETERS; OXIDE-INDUCED APOPTOSIS; BCL-2; FAMILY-MEMBERS; SMOOTH-MUSCLE-CELLS; OLD FEMALE RATS; BODY-COMPOSITION; NITRIC-OXIDE;

D O I：

10.1093/gerona/glr083

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

The effect of a chronic combined treatment with growth hormone (GH) plus melatonin (Mel) on different age-related processes in cytosolic and nuclear fractions of hearts from SAMP8 mice (2 and 10 months) has been investigated. The parameters studied have been messenger RNA expressions of IL-1, IL-10, NFkBp50, NFkBp52, TNF alpha, eNOS, iNOS, HO-1, HO-2, BAD, BAX, and Bcl2 and protein expressions of iNOS, eNOS, TNF alpha, IL-1, IL-10, NFkBp50, NFKbp52, and caspase activity (3 and 9). Our results supported the existence of a proapoptotic and oxidative status together with inflammatory processes in the heart of old mice, with increases of inflammatory cytokines, caspase activity, HO-1, BAX, NFkBp50, and NFkBp52 and decreases of eNOS and Bcl2. Also, we were able to observe the translocation of NFkB to nuclei. The combined treatment was able to partially reduce the incidence of these deleterious changes, showing differences with the separated treatments with GH and Mel as were investigated in previous articles from our group.

引用

页码：823 / 834

页数：12

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