The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex

被引：254

作者：

Verduci, Lorena ^{[1
]}

Ferraiuolo, Maria ^{[1
,2
]}

Sacconi, Andrea ^{[1
]}

Ganci, Federica ^{[1
]}

Vitale, Jlenia ^{[1
]}

Colombo, Teresa ^{[3
]}

Paci, Paola ^{[4
]}

Strano, Sabrina ^{[2
]}

Macino, Giuseppe ^{[5
]}

Rajewsky, Nikolaus ^{[6
]}

Blandino, Giovanni ^{[1
]}

机构：

[1] Italian Natl Canc Inst, Oncogen & Epigenet Unit, I-00144 Rome, Italy

[2] Italian Natl Canc Inst, Mol Chemoprevent Unit, I-00144 Rome, Italy

[3] CNR, Inst Comp Applicat Mauro Picone, I-00185 Rome, Italy

[4] CNR, Inst Syst Anal & Comp Sci Antonio Ruberti, I-00185 Rome, Italy

[5] Univ Roma La Sapienza, Dept Cellular Biotechnol & Hematol, I-00161 Rome, Italy

[6] Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Syst Biol Gene Regulatory Elements, D-13125 Berlin, Germany

来源：

GENOME BIOLOGY | 2017年 / 18卷

关键词：

circPVT1; mut-p53; PVT1; YAP; TEAD; miR-497-5p; HNSCC; CIRCULAR RNA; MUTATIONAL LANDSCAPE; CANCER; GROWTH; P53; EXPRESSION; BIOMARKER; BIOGENESIS; OVARIAN;

D O I：

10.1186/s13059-017-1368-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Background: Circular RNAs are a class of endogenous RNAs with various functions in eukaryotic cells. Worthy of note, circular RNAs play a critical role in cancer. Currently, nothing is known about their role in head and neck squamous cell carcinoma (HNSCC). The identification of circular RNAs in HNSCC might become useful for diagnostic and therapeutic strategies in HNSCC. Results: Using samples from 115 HNSCC patients, we find that circPVT1 is over-expressed in tumors compared to matched non-tumoral tissues, with particular enrichment in patients with TP53 mutations. circPVT1 up-and down-regulation determine, respectively, an increase and a reduction of the malignant phenotype in HNSCC cell lines. We show that circPVT1 expression is transcriptionally enhanced by the mut-p53/YAP/TEAD complex. circPVT1 acts as an oncogene modulating the expression of miR-497-5p and genes involved in the control of cell proliferation. Conclusions: This study shows the oncogenic role of circPVT1 in HNSCC, extending current knowledge about the role of circular RNAs in cancer.

引用

页数：24

共 61 条

[1]

Exome Sequencing of Head and Neck Squamous Cell Carcinoma Reveals Inactivating Mutations in NOTCH1 [J].

Agrawal, Nishant ;

Frederick, Mitchell J. ;

Pickering, Curtis R. ;

Bettegowda, Chetan ;

Chang, Kyle ;

Li, Ryan J. ;

Fakhry, Carole ;

Xie, Tong-Xin ;

Zhang, Jiexin ;

Wang, Jing ;

Zhang, Nianxiang ;

El-Naggar, Adel K. ;

Jasser, Samar A. ;

Weinstein, John N. ;

Trevino, Lisa ;

Drummond, Jennifer A. ;

Muzny, Donna M. ;

Wu, Yuanqing ;

Wood, Laura D. ;

Hruban, Ralph H. ;

Westra, William H. ;

Koch, Wayne M. ;

Califano, Joseph A. ;

Gibbs, Richard A. ;

Sidransky, David ;

Vogelstein, Bert ;

Velculescu, Victor E. ;

Papadopoulos, Nickolas ;

Wheeler, David A. ;

Kinzler, Kenneth W. ;

Myers, Jeffrey N. .

SCIENCE, 2011, 333 (6046) :1154-1157

[2]

Circular RNA enrichment in platelets is a signature of transcriptome degradation [J].

Alhasan, Abd A. ;

Izuogu, Osagie G. ;

Al-Balool, Haya H. ;

Steyn, Jannetta S. ;

Evans, Amanda ;

Colzani, Maria ;

Ghevaert, Cedric ;

Mountford, Joanne C. ;

Marenah, Lamin ;

Elliott, David J. ;

Santibanez-Koref, Mauro ;

Jackson, Michael S. .

BLOOD, 2016, 127 (09) :E1-E11

[3]

circRNA Biogenesis Competes with Pre-mRNA Splicing [J].

Ashwal-Fluss, Reut ;

Meyer, Markus ;

Pamudurti, Nagarjuna Reddy ;

Ivanov, Andranik ;

Bartok, Osnat ;

Hanan, Mor ;

Evantal, Naveh ;

Memczak, Sebastian ;

Rajewsky, Nikolaus ;

Kadener, Sebastian .

MOLECULAR CELL, 2014, 56 (01) :55-66

[4]

Balz V, 2003, CANC RES

[5]

Long-Term Quality of Life After Total Laryngectomy and Postoperative Radiotherapy Versus Concurrent Chemoradiotherapy for Laryngeal Preservation [J].

Boscolo-Rizzo, Paolo ;

Maronato, Francesca ;

Marchiori, Carlo ;

Gava, Alessandro ;

Da Mosto, Maria Cristina .

LARYNGOSCOPE, 2008, 118 (02) :300-306

[6]

Broad Institute TCGA Genome Data Analysis Center, 2016, AN READ STAND TCGA D

[7]

Whole genome RNA expression profiling of endoscopic biliary brushings provides data suitable for biomarker discovery in cholangiocarcinoma [J].

Chapman, Michael H. ;

Tidswell, Robert ;

Dooley, James S. ;

Sandanayake, Neomal S. ;

Cerec, Virginie ;

Deheragoda, Maesha ;

Lee, Alvin J. X. ;

Swanton, Charles ;

Andreola, Fausto ;

Pereira, Stephen P. .

JOURNAL OF HEPATOLOGY, 2012, 56 (04) :877-885

[8]

Circular RNA profile identifies circPVT1 as a proliferative factor and prognostic marker in gastric cancer [J].

Chen, Jie ;

Li, Yan ;

Zheng, Qiupeng ;

Bao, Chunyang ;

He, Jian ;

Chen, Bin ;

Lyu, Dongbin ;

Zheng, Biqiang ;

Xu, Yu ;

Long, Ziwen ;

Zhou, Ye ;

Zhu, Huiyan ;

Wang, Yanong ;

He, Xianghuo ;

Shi, Yingqiang ;

Huang, Shenglin .

CANCER LETTERS, 2017, 388 :208-219

[9]

Chou T.C., 2005, CompuSyn for drug combinations: PC software and users guide: a computer program for quantitation of synergism and antagonism in drug combinations, and the determination of IC50 and ED50 and LD50 values

[10]

MISSPLICING YIELDS CIRCULAR RNA MOLECULES [J].

COCQUERELLE, C ;

MASCREZ, B ;

HETUIN, D ;

BAILLEUL, B .

FASEB JOURNAL, 1993, 7 (01) :155-160

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