Cell-free translation reconstituted with purified components

被引：1404

作者：

Shimizu, Y

Inoue, A

Tomari, Y

Suzuki, T

Yokogawa, T

Nishikawa, K

Ueda, T

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Kashiwa, Chiba 2778562, Japan

[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Kashiwa, Chiba 2778562, Japan

[3] Gifu Univ, Fac Engn, Dept Biomol Sci, Gifu 5011193, Japan

来源：

NATURE BIOTECHNOLOGY | 2001年 / 19卷 / 08期

基金：

日本学术振兴会;

关键词：

D O I：

10.1038/90802

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 mug/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).

引用

页码：751 / 755

页数：5

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