Quantitative expression patterns of peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) protein in mice

被引：134

作者：

Girroir, Elizabeth E. ^{[1
]}

Hollingshead, Holly E. ^{[1
,2
]}

He, Pengfei ^{[1
]}

Zhu, Bokai ^{[1
]}

Perdew, Gary H. ^{[1
]}

Peters, Jeffrey M. ^{[1
,2
]}

机构：

[1] Penn State Univ, Ctr Mol Toxicol & Carcinogenesis, Dept Vet & Biomed Sci, University Pk, PA 16802 USA

[2] Penn State Univ, Grad Program Biochem Microbiol & Mol biol, University Pk, PA 16802 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 371卷 / 03期

关键词：

peroxisome proliferator-activated receptor beta/delta; antibody; expression; biochemistry;

D O I：

10.1016/j.bbrc.2008.04.086

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The expression patterns of PPAR beta/delta have been described, but the majority of these data are based on mRNA data. To date, there are no reports that have quantitatively examined the expression of PPAR beta/delta protein in mouse tissues. In the present study, a highly specific PPAR beta/delta antibody was developed, characterized, and used to examine tissue expression patterns of PPAR beta/delta. As compared to commercially available anti-PPAR beta/delta antibodies, one of six polyclonal anti-PPAR beta/delta antibodies developed was significantly more effective for immunoprecipitation of in vitro-translated PPAR beta/delta. This antibody was used for quantitative Western blot analysis using radioactive detection methods. Expression of PPAR beta/delta was highest in colon, small intestine, liver, and keratinocytes as compared to other tissues including heart, spleen, skeletal muscle, lung, brain, and thymus. Interestingly, PPAR beta/delta expression was localized in the nucleus and RXR alpha can be co-immunoprecipitated with nuclear PPAR beta/delta. Results from these studies demonstrate that PPAR beta/delta expression is highest in intestinal epithelium, liver, and keratinocytes, consistent with significant biological roles in these tissues. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：456 / 461

页数：6

共 17 条

[1]

Differential expression of peroxisome proliferator-activated receptor-α, -β, and -γ during rat embryonic development [J].