Testing for endocytosis in plants

被引:33
作者
Aniento, F
Robinson, DG
机构
[1] Heidelberg Univ, Heidelberg Inst Plant Sci, D-69120 Heidelberg, Germany
[2] Univ Valencia, Fac Farm, Dept Bioquim & Biol Mol, E-46010 Valencia, Spain
关键词
biotinylated marker; FM lipophilic styryl dye; Lucifer Yellow; tyrphostin; wortmannin;
D O I
10.1007/s00709-005-0101-y
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
For many years endocytosis has been regarded with great scepsis by plant physiologists. Although now generally accepted, care must still be taken with experiments designed to demonstrate endocytic uptake at the plasma membrane. We have taken a critical look at the various agents which are in use as markers for plant endocytosis, pointing out pitfalls and precautions which should be taken. We also take this opportunity to introduce the tyrphostins - tyrosine kinase inhibitors -, which also seem to prevent endocytosis in plants.
引用
收藏
页码:3 / 11
页数:9
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