Specific loss of the extrinsic 18 KDa protein from Photosystem II upon heating to 47°C causes inactivation of oxygen evolution likely due to Ca release from the Mn-complex

被引：30

作者：

Barra, M ^{[1
]}

Haumann, M ^{[1
]}

Dau, H ^{[1
]}

机构：

[1] Free Univ Berlin, FB Phys, D-14195 Berlin, Germany

来源：

PHOTOSYNTHESIS RESEARCH | 2005年 / 84卷 / 1-3期

关键词：

calcium; extrinsic polypeptides; oxygen evolution; Photosystem II; temperature jump;

D O I：

10.1007/s11120-004-7158-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Exposure of Photosystem II (PS II) membrane particles from spinach to a temperature of 47 degrees C caused the rapid release of the 18 kDa protein in parallel to inactivation of oxygen evolution. Previously, it has been suggested that the first heat-jump response involves rapid Ca release from the Mn complex of O-2-evolution, followed by the slower release of (2 + 2) Mn(II)ions [Pospisil P et al. (2003) Biophys J 84: 1370-1386]. Here, the predicted biphasic MnII release to the bulk was verified by atomic absorption spectroscopy (AAS). Analysis of laser flash-induced delayed fluorescence transients suggests that the loss of the essential Ca ion from the Mn4Ca complex in the dark is due to the loss of the 18 kDa protein. The S-2-state multiline EPR signal of the Mn complex was still generated in heat-treated PS II presumably lacking Ca, but retaining four Mn ions.

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页码：231 / 237

页数：7

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