Preparation of N2,N2,7-trimethylguanosine affinity columns

被引：3

作者：

Espuny, R ^{[1
]}

Bahia, D ^{[1
]}

Cicarelli, RMB ^{[1
]}

Codony, C ^{[1
]}

Khaouja, A ^{[1
]}

Aviñó, AM ^{[1
]}

Eritja, R ^{[1
]}

Bach-Elias, M ^{[1
]}

机构：

[1] CSIC, Ctr Invest & Desarrollo, Barcelona 08034, Spain

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1999年 / 18卷 / 01期

关键词：

D O I：

10.1080/07328319908045599

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

2,2,7-trimethylguanosine (TMG) binding proteins from human cells were purified through TMG-affinity columns. TMG synthesis was improved and the TMG obtained was shown to be similar to the TMG in the 5' cap of the UsnRNAs. The eluates obtained with TMG-affinity chromatographies were very different from those isolated with m(7)G-affinity columns, thus suggesting that specific TMG-binding proteins were obtained. The fraction may be enriched with factors associated with import and/or hypermethylation of UsnRNPs.

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页码：125 / 136

页数：12

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