The Pseudomonas fluorescens SBW25 wrinkly spreader biofilm requires attachment factor, cellulose fibre and LIPS interactions to maintain strength and integrity

被引:99
作者
Spiers, AJ
Rainey, PB
机构
[1] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
[2] Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand
来源
MICROBIOLOGY-SGM | 2005年 / 151卷
关键词
D O I
10.1099/mic.0.27984-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The wrinkly spreader (WS) isolate of Pseudomonas fluorescens SBW25 forms a substantial biofilm at the air-liquid interface. The biofilm is composed of an extracellular partially acetylated cellulose-fibre matrix, and previous mutagenesis of WS with mini-Tn5 had identified both the regulatory and cellulose-biosynthetic operons. One uncharacterized WS mutant, WS-5, still expressed cellulose but produced very weak biofilms. In this work, the mini-Tn5 insertion site in WS-5 has been identified as being immediately upstream of the to/-pal operon. Like Tol-Pal mutants of other Gram-negative bacteria, WS-5 showed a 'leaky-membrane' phenotype, including the serendipitous ability to utilize sucrose, increased uptake of the hydrophilic dye propidium iodide, and the loss of lipopolysaccharide (LPS) expression. WS-5 cells were altered in relative hydrophobicity, and showed poorer recruitment and maintenance in the biofilm than WS. The WS-5 biofilm was also less sensitive to chemical interference during development. However, growth rate, cellulose expression and attachment were not significantly different between WS and WS-5. Finally, WS-5 biofilms could be partially complemented with WS-4, a biofilm- and attachment-deficient mutant that expressed LPS, resulting in a mixed biofilm with significantly increased strength. These findings show that a major component of the WS air-liquid biofilm strength results from the interactions between LPS and the cellulose matrix of the biofilm - and that in the WS biofilm, cellulose fibres, attachment factor and LPS are required for biofilm development, strength and integrity.
引用
收藏
页码:2829 / 2839
页数:11
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