Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using MSn spectral matching

被引：34

作者：

Takegawa, Y

Ito, S

Yoshioka, S

Deguchi, K ^{[1
]}

Nakagawa, H

Monde, K

Nishimura, SI

机构：

[1] Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0010021, Japan

[2] Hitachi High Technol Co, Hitachinaka 3128504, Japan

[3] Hitachi Sci Syst Co, Naka Customer Ctr, Hitachinaka 3128504, Japan

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 04期

关键词：

D O I：

10.1002/rcm.1341

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two isomeric pairs of 2-aminopyridine (PA)-derivatized fucosylated and non-fucosylated oligosaccharides (complex-type N-glycans of IgG) were analyzed using liquid chromatography/ion trap mass spectrometry (LC/ITMS) with a sonic spray ionization source and by varying the collision-induced dissociation voltage. Reproducibility of MSn (n = 2) spectra obtained by LC/ITMS was tested considering both fragment ions (m/z) and intensities. A comparison of their MSn spectra and evaluation of similarities (or matching), based on correlation coefficients between MSn spectra, was investigated as a possibility for structural assignment of the isomers. It is shown that such MSn spectral matching is useful and applicable to the structural assignment of relatively large fucosylated and sialylated PA-oligosaccharides released from IgG based on Bn- and Yn-type fragmentations of the corresponding [M+H+Na](2+) ions. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：385 / 391

页数：7

共 29 条

[1] Branching pattern and sequence analysis of underivatized oligosaccharides by combined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry [J].