Pulsed electrochemical technique for monitoring antibody-antigen reactions at interfaces

被引：17

作者：

Sargent, A ^{[1
]}

Sadik, OA ^{[1
]}

机构：

[1] SUNY Binghamton, Dept Chem, Binghamton, NY 13902 USA

来源：

ANALYTICA CHIMICA ACTA | 1998年 / 376卷 / 01期

关键词：

immunosensors; antibody-antigen interactions; pulsed amperometric detection; reversibility;

D O I：

10.1016/S0003-2670(98)00450-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The mechanism of pulsed potential waveform for monitoring antibody-antigen interactions at immunosensor interfaces is discussed. Some examples of antibody-antigen interactions at quartz crystal microbalance and polymer-modified antibody electrodes are presented. The binding and release of analytes to the modified sensor surfaces are monitored in real time. An overview of the analytical performance of some recently developed sensors utilizing pulsed amperometric detection techniques is also presented. The sensors obtained are reversible and reproducible (with a relative standard deviation of 0.30%), and their response times are in the millisecond range. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：125 / 131

页数：7

共 24 条

[1] DETERMINATION OF P-CRESOL (AND OTHER PHENOLICS) USING A CONDUCTING POLYMER-BASED ELECTRO-IMMUNOLOGICAL SENSING SYSTEM
BARNETT, D
LAING, DG
SKOPEC, S
SADIK, O
WALLACE, GG
[J]. ANALYTICAL LETTERS, 1994, 27 (13) : 2417 - 2429
[2] BIOTIEUX JL, 1984, ANAL CHIM ACTA, V63, P308
[3] REGENERABLE FIBER-OPTIC-BASED IMMUNOSENSOR
BRIGHT, FV
BETTS, TA
LITWILER, KS
[J]. ANALYTICAL CHEMISTRY, 1990, 62 (10) : 1065 - 1069
[4] COLINS WP, 1985, ALTERNATIVE IMMUNOAS, P103
[5] DEBRUIJN HE, 1992, APPL OPTICS, V31, P440, DOI 10.1364/AO.31.0440_1
[6] EUROPIUM CHELATE LABELS IN TIME-RESOLVED FLUORESCENCE IMMUNOASSAYS AND DNA HYBRIDIZATION ASSAYS
DIAMANDIS, EP
CHRISTOPOULOS, TK
[J]. ANALYTICAL CHEMISTRY, 1990, 62 (22) : A1149 - +
[7] DOYL JM, 1987, ELECTROCHEMICAL SENS, P87
[8] Engvall E, 1980, Methods Enzymol, V70, P419
[9] GOSLING JP, 1990, CLIN CHEM, V36, P1408
[10] SURFACE-PLASMON RESONANCE IMMUNOSENSORS - SENSITIVITY CONSIDERATIONS
KOOYMAN, RPH
KOLKMAN, H
VANGENT, J
GREVE, J
[J]. ANALYTICA CHIMICA ACTA, 1988, 213 (1-2) : 35 - 45

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